Nano/micro roughness

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Table of contents

Chapter 1 – Introduction
1 Integrin binding to extracellular matrix, a model for adhesion on biomaterials
2 Stimuli-responsive biomaterials to modulate in situ the effects of adhesive cues
3 Coating based on PLL-g-P(NIPAM-co-RGD)
Bibliography
Chapter 2 – Synthetic route to PLL-g-PNIPAM derivatives
Introduction
1 PLL functionalization
2 Synthesis of -Ci,-NHS-P(NIPAM-co-B) grafts
Conclusion
Bibliography
Chapter 3 – Characterizations of surfaces coated with PLL-g-PNIPAM derivatives
Introduction
1 Adsorption of PLL-g-PNIPAM on flat SiO2 surfaces
2 Thermal transition of PLL-g-PNIPAM coatings
Conclusions and discussion on thermal transition of PLL-g-PNIPAM adlayers
Bibliography
Chapter 4 – Temperature-controlled ligand accessibility in PLL-g-PNIPAM coatings
Introduction
1 Effect of ligand position in PLL-g-PNIPAM
2 Effect of ligand density
3 Effect of the size of Streptavidin probes
Conclusion
Bibliography
Chapter 5 – Cell adhesion on PLL-g-PNIPAM coatings
Introduction
1 Pure (100 %) PLL-g-[0.15](NIPAM-co-RGD)(6) adlayers
2 Cell adhesion on mixed PLL-g-[0.2]PNIPAM(6)/PLL-g-[0.15]P(NIPAM-co-RGD)(6) coatings at 27
and 37 °C
3 Cell detachment from mixed PLL-g-[0.2]PNIPAM(6)/PLL-g-[0.15]P(NIPAM-co-RGD)(6) coatings
4 An example of dynamic experiment on mixed thermo-responsive PLL-g-[0.15]P(NIPAM-co-RGD)(6)/PLL-g-[0.2]PNIPAM(6) coatings
Conclusion
Bibliography
Chapter 6 – Studies of poly(N-isopropylacrylamide) polymers functionalized with azobenzene to develop light-responsive brushes
Introduction
1 State of the art on thermo- and photo-responsive polymers
2 Our strategy to make our PNIPAM grafts sensitive to light
3 Studies of PNIPAM copolymers with azobenzene pendant groups
4 Studies of PNIPAM copolymers with azobenzene at one extremity
5 Use of azobenzene-containing PNIPAM as light-responsive polymer coatings
Conclusion
Bibliography
Conclusion & perspectives
Appendix 1 – Protocols for synthesis
1 Materials and methods
2 Synthesis of HOOC-Azo-NH2
3 Synthesis of -Ci,-NHS-P(NIPAM-co-B) and -Ci,-COOH-P(NIPAM-co-B)
4 Grafting of NHS-compounds on PLL
Appendix 2 – Coating and patterning with PLL derivatives
1 Coating of coverslips with PLL derivatives
2 Photolithography to obtain micrometric patterns of PLL derivatives
Appendix 3 – PNIPAM phase transition
1 PNIPAM phase transition in aqueous solution
2 Phase transition of PLL-g-PNIPAM adlayers
Bibliography
Appendix 4 – Captures of fluorescent probes on PLL-g-PNIPAM patterns
1 Generic protocol used for captures of fluorescent probes
2 Adsorption of Streptavidin-Cy3 on mixed PLL-g-[0.1]P(NIPAM-co-biotin)(6):PLL-g-PEG adlayers
3 Non-specific protein adsorption on PLL-g-[0.2]PNIPAM(6)
Appendix 5 – Cell experiments
1 Cell seeding
2 Qualitative experiments of cell adhesion
3 Quantification of cell adhesion
4 Cell detachment
5 Reversible temperature-triggered change in cell shape
Appendix 6 – Thermal relaxation