Isolation and multiplication of nematodes

For more info about our services contact : help@bestpfe.com

Table of contents

Introduction
Material and methods
Plant and fungal material
Germination of seeds
Bacteria
Nematodes
-Isolation and multiplication of nematodes
-Sterilization and testing of nematodes with P.fluorescens
Co-inoculation experiment
Measurements
Root parameters
Nematodes counting
Nitrogen cycling
Presentation of results and statistical analysis
Results
Assimilation of NH4 in bacterial biomass
Plant biomass
Shoot dry weight
Root architecture
Root tips, forks, surface area and length parameters
Number of nematodes at the end of experiment
Nitrogen accumulation in shoots
15N accumulation in shoot biomass
Discussions
Effects of co-inoculation treatments on shoot biomass and root development of P. pinaster seedlings
Effects of bacteria alone and with fungi on nematodes population in rhizosphere of pine plant
Effect of inoculation treatments on total N and 15N accumulation by shoot biomass