Dissolved oxygen concentration

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Table of contents

INTRODUCTION AND CONTEXT OF THE STUDY
PART I: LITERATURE REVIEW
I.1 Bioreactor heterogeneities and bioperformances
I.1.1 Origin of heterogeneities inside bioreactor
I.1.2 Nature of heterogeneities in industrial bioreactors
I.1.3 Impact on bioprocess performances
I.2 Physiological responses of Yarrowia lipolytica to stress conditions
I.2.1 General description of Yarrowia lipolytica
I.2.2 Morphological changes in Yarrowia lipolytica: dimorphism phenomena
I.2.3 Metabolic responses of Yarrowia lipolytica to adverse environmental conditions
I.2.4 Mechanisms regulating stress responses in Yarrowia lipolytica
I.3 Conclusions and objectives of the study
PART II: MATERIALS AND METHODS
II.1 Strain and culture media
II.1.1 Strain
II.1.2 Culture media
II.2 Culture conditions
II.2.1 Strain preservation
II.2.2 Inoculum preparation
II.2.3 Bioreactor fermentation
II.3 Model particles
II.3.1 Calibration particles: from 1 to 15 μm-diameter
II.3.2 Calibration particles: 40 and 80 μm-diameter
II.4 Analytical methods
II.4.1 Biomass characterization
II.4.2 Determination of glucose and organic acids concentrations
II.4.3 Gas analysis
II.5 Methodology for data treatment
II.5.1 Rates expression for gas-phase reactions
II.5.2 Rate expressions for liquid-phase reactions
II.5.3 Determination of overall yields
II.5.4 Carbon and redox balances
II.5.5 Smoothing and reconciliation of the experimental data
PART III: RESULTS AND DISCUSSION
Chapter III-1: Development and validation of methodologies for the quantification and characterization of morphological changes in Yarrowia lipolytica
III.1.1 Introduction
III.1.2 Experimental strategy
III.1.3 Results and discussion
III.1.4 Conclusion
III.1.5 Results synthesis
Chapter III-2: Characterization of the mycelial transition of Yarrowia lipolytica by morphogranulometric measurements
III.2.1 Introduction
III.2.2 Experimental strategy
III.2.3 Results and discussion
III.2.4 Conclusions
III.2.5 Results synthesis
Chapter III-3: Dynamic behavior of Yarrowia lipolytica in response to pH perturbations: dependence of the stress response on the culture mode
III.3.1 Introduction
III.3.2 Publication: Dynamic behavior of Yarrowia lipolytica in response to pH perturbations: dependence of the stress response on the culture mode
III.3.3 Results synthesis
Chapter III-4: Investigation of the effect of oxygen availability on the metabolism and morphology of Yarrowia lipolytica: more insights into the impact of glucose levels on dimorphism
III.4.1 Introduction
III.4.2 Publication: Investigation of the effect of oxygen availability on the metabolism and morphology of Yarrowia lipolytica: more insights into the impact of glucose levels on dimorphism
III.4.3 Results synthesis
GENERAL DISCUSSION, CONCLUSIONS AND PERSPECTIVES
REFERENCES