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Table of contents
Chapter I
Chapter II (Article I)
Short resume
Abstract
1. Introduction
2. Materials and methods
2.1. Animals
2.2. Immunoblots
2.3. RNA extraction and RT-qPCR
2.4. Mixed cell culture
2.5. Immunoisolation and culture of CNS neurons from postnatal mice
2.6. Immunocytochemistry
2.7. Statistical analysis
3. Results
3.1. Lsr RNA profiling in mice brain
3.2. LSR differential expression in different brain regions and the retina
3.1. LSR expression in neurons and glial cells
3.2. Glial cells highly express LSR in cerebellum
4. Conclusions and discussion
5. Additional data and supporting information
Chapter III
Short resume
Resume
1. Introduction
1.1. Scientific question
1.2. Goal of the study
2. Materials and methods
2.1. Animals
2.2. Breeding and generation of experimental groups
2.3. Genotyping of Cre and floxed lsr mice
2.4. Tamoxifen (TAM) induction of GlastCreERT2 enzyme
2.5. Behavioral tests
2.6. RNA extraction and RT-qPCR
2.7. Blood glucose test
2.8. Statistical analysis
3. Results and conclusions
3.1. Lsr downregulation in cKO mice
3.2. Activity and anxiety
3.3. Olfaction
3.4. Memory
4. Discussion and perspectives
5. Supplementary data
6. Preliminary results
6.1. Lsr excision from hippocampus, cerebellum, and olfactory bulb
6.2. Effects of glia-specific lsr excision on cholesterol metabolism
Chapter IV
Short resume
1. Introduction
2. Results
2.1. Home cage activity
2.2. Free exploratory paradigm
2.3. Open field test
2.4. Buried cookie test
2.5. Odor discrimination test
2.6. Object recognition test
2.7. Three-chambered sociability test
2.8. Y-maze
2.9. Barnes maze
3. Conclusion and discussion
General discussion
