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Table of contents
Chapter I. General introduction
A. General overview
B. Physical barriers and immune signaling responses as defense mechanisms against microbial pathogens
C. Impact of heat stress on plant immunity
Review article: Fight hard or die trying: plants versus pathogens under heat stress
D. The lofty goal of identifying uncovered resistance mechanisms under heat stress
1. The pathosystem Arabidopsis thaliana – Ralstonia solanacearum
a. Arabidopsis thaliana
b. Ralstonia solanacearum
c. Arabidopsis thaliana and Ralstonia solanacearum interactions
2. Exploring natural diversity of plants to identify the genetic basis of resistance
a. Traditional QTL mapping
b. Genome wide association mapping (GWA)
E. Thesis project objectives
Chapter II. Identification of the genetic basis of natural variation of plant response to Ralstonia solanacearum under elevated temperature in a worldwide population of Arabidopsis
A. Introduction
B. Genetic basis of early resistance to Ralstonia solanacearum under elevated temperature
Article: Quantitative Disease Resistance Under Elevated Temperature: Genetic Basis of New Resistance
Mechanisms to Ralstonia solanacearum
Supporting information
C. Naturally resilient: the genetic basis of total resistance to Ralstonia solanacearum remaining efficient at elevated temperature
1. Introduction
2. Material and methods
Bacterial strains, plant materials and growth conditions
Plant inoculation and phenotyping
Genetic architecture, bulk segregant analysis, DNA extraction and sequencing
SNP mapping analysis
RPS4/RRS1-REden-1 locus cloning and analysis of polymorphisms
Hypersensitive Response assays
3. Results
EDEN-1: robust resistance to GMI1000 at 30°C
Genetic architecture of Eden-1 total resistance to GMI1000 at 30°C
Heat-stable resistance of Eden-1 is dependent on perception of PopP2
Eden-1 genome contains a specific allelic version of RPS4-RRS1-R
Heat-stable resistance of Eden-1 is conferred by RPS4/RRS1-REden-1
4. Discussion
D. Conclusion
Chapter III. Identification of the genetic basis of natural variation of plant response to Ralstonia solanacearum under elevated temperature in a local population of Arabidopsis
A. Introduction
B. Article: Natural variation of Arabidopsis thaliana quantitative disease response to Ralstonia solanacearum is controlled by a complex genetic architecture involving additive and epistatic QTLs
C. Supporting information
D. Discussion and conclusion
Chapter IV. Discussion and Perspectives
Discussion
Perspectives
References
