The impact of packaging yeast cells as “YCW”

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Table of contents

Chapter I: LITERATURE REVIEW
1. Generality on yeast
1.1. Physiology: generality about growth, metabolism and genetic
1.2. Yeast and the mode of culture and production process
1.3. Cell wall as biotechnological product
2. Methods to study the yeast cell wall
2.1. Methods to determine the yeast cell wall composition
2.2. Biophysical approach: Atomic Force Microscopy
2.3. Molecular tools: transcription profile and genomic analysis
2.4. Phenotypic observations: use of drugs
3. Biosynthesis of the cell wall polysaccharides
3.1. Chitin
3.2. β-1,3-glucan
3.3. β-1,6-glucan
3.4. Mannoproteins
4. Architecture of the cell wall and remodeling enzymes
4.1. Architecture of the yeast cell wall
4.2. Hydrolytic enzymes of the yeast cell wall
4.3. Signalling pathways involved in the yeast cell wall architecture
5. Conclusion of the literature review and objectives of the thesis
Chapter II: DETERMINATION OF THE YEAST CELL WALL COMPOSITION
Chapter III: EFFECT OF THE STRAIN BACKGROUND ON THE CELL WALL
Chapter IIIA: STUDY OF CELL WALL MUTANTS (gas1Δ, chs3Δ, mnn9Δ)
Chapter IIIB: STUDY OF FOUR INDUSTRIAL STRAINS
Supplementary results: Cell wall composition and transcriptomic analysis of industrial yeasts: Effect of the growth medium
Discussion of the results
Chapter IV: IMPACT OF AUTOLYSIS PROCESS ON THE CELL WALL
Chapitre V: CONCLUSION AND PERSPECTIVES
1. Conclusion
2. Perspectives
Chapter VI: REFERENCES
Chapter VII: APPENDIX
1. List of figures
2. List of tables
3. Abbreviations
4. Material and methods
4.1. Strains
4.2. Proteins measurement
4.3. Analysis of sugars : HPAEC-PAD
4.4. Production of endo-β(1,6)-glucanase from Trichoderma harzianum
4.5. Atomic force microscopy
4.6. Canonical correlations of cell wall physico-chemical traits and transcriptomic profile
5. List of Publications
6. List of Communications