NMR and mass spectrometry

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Table of contents

CHAPTER I
Abstract
1. Introduction
2. Materials and methods
2.1. Plant material and reagents
2.2. Extraction and isolation procedures
2.3. NMR and mass spectrometry
2.4. Cell culture and cell viability assay
2.5. Statistical analysis
3. Results and discussion
4. Conclusion
5. References
CHAPTER II
Abstract
1. Introduction
2. Materials and methods
2.1 Stilbene compounds
2.2 Reagents and material
2.3 Cell culture
2.4 Cytoxicity assays
2.5 Measurement of NO production
2.6 Measurement of ROS production
2.7 Measurement of TNF‐α and IL‐1β production
2.8 Statistical analysis
3. Results
4. Discussion
5. References
CHAPTER III
Abstract
1. Introduction
2. Materials and methods
2.1. Stilbenes from Vitis Vinifera
2.2. Cell Culture
2.3. Cell Viability Assay
2.4. Cell Cycle Analysis
2.5. Intracellular ROS and Mitochondrial O2− Measurement
2.6. Caspase‐3 Activity
2.7. Western Blot Analysis
2.8. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
6. References
CHAPTER IV
Abstract
1. Introduction
2. Materials and methods
2.1. Reagents and antibodies
2.2. Stilbene extraction from Vitis vinifera
2.3. Cell cultures
2.4. Colony formation assay
2.5. Transwell migration assay
2.6. Transient p53 silencing in HepG2 cells
2.7. Transient p53 transfection into Hep3B cells
2.8. Western blot analysis
2.9. Lactate dehydrogenase release assay
2.10. Hydrogen peroxide measurement
2.11. Statistical analysis
3. Results
4. Discussion
5. References