(Downloads - 0)
For more info about our services contact : help@bestpfe.com
Table of contents
INTRODUCTION
1. Legume crops in sustainable agriculture and ecology
2. Symbiotic association engaging plants and nitrogen-fixing bacteria
3. Nodule shapes and evolution in the Rosid I clade
4. Indeterminate versus determinate legume nodules
5. Nod factors signaling-dependent activation of nodule organogenesis
6. Symbiotic organ identity regulation
7. Gene networks controlling biogenesis of shoot apical meristem (SAM), axillary meristems (AMs), and floral meristems (FMs)
8. The NBCL genes in lateral organ boundary regulation
9. The role of NBCLs in leaf formation and patterning
10. The NBCL genes control flowering-time, inflorescence architecture and internode patterning
11. The role of NBCL genes in floral patterning and symmetry
12. The NBCL genes involved in shoots and inflorescence branching
13. The role of BOP in fruit architecture and lignin biosynthesis
14. NBCLs are essential for differentiation and separation of abscission in dicot
15. BOPs interact with other factors to mainten development
16. The other roles of BOPs
CHAPTER I. Legume NBCLs genes are redundantly required for aerial organ development and root nodule identity
Abstract
INTRODUCTION
RESULTS
MtNOOT1 and MtNOOT2 genes expression in M. truncatula aerial organs
PsCOCH1 and PsCOCH2 are co-expressed in aerial organ and are induced in indeterminate nodules of P. sativum
MtNOOT1 and MtNOOT2 redundantly control stipule development
PsCOCH2 mutation increased the PsCOCH1 aerial vegetative mutant phenotypes
NBCL genes are important for plant architecture
MtNOOT1 and MtNOOT2 are required for flower development
Pscoch1coch2 present accentuated floral patterning
Legume NBCLs control pod number and seed size
PsCOCH2 participates to the symbiotic organ development and functioning (this part from Kevin Magne PhD thesis)
NBCL2 participates to the regulation of the floral patterning in M. truncatula and pea
The NBCL clade shares conserved function governing fruit architecture
PsCOCH2 is involved in nodule development and identity
MATERIALS AND METHODS
Plant material
Coch2 mutant isolation
Plant growth conditions
Plant genotyping
Material fixation and X-gluc staining
RNA preparation and reverse transcription
qRT-PCR gene expression analysis
Acetylene reduction assay
CHAPTER II. The COCHLEATA1 gene controls branching and flowering time in pea
Abstract
INTRODUCTION
RESULTS
Mutations in the pea COCH1 gene increase shoot branching
In Medicago Mtnoot1 and Mtnoot2 play opposite roles in lateral branching
The COCH1 gene is necessary for long-distance signaling
PsCOCH1 is deficient in the SL signaling pathway
PsCOCH1 expression is downregulated by CK and responds to exogenous CK application independently of SL
IAA stimulates PsCOCH1 expression
The legume NBCLs participate in flowering time determination
DISCUSSION
A novel role for NBCL genes in plant development
PsCOCH1 is necessary for long-distance signaling and deficient in the SL signaling pathway .
PsCOCH1 participates in hormone cross talk to control plant architecture
NBCL genes are involved in flowering-time regulation
Extending the concept of strigolactone in floral transition and nodule identity
MATERIAL AND METHODS
Plant material, growth conditions and scoring methods
Grafting studies
Strigolactone application
Exogenous auxin studies
RNA extraction and cDNA synthesis
CHAPTER III. The Brachypodium distachyon BLADE-ON-PETIOLE-Like proteins UNICULME4 and LAXATUM-A are redundantly required for plant development
Summary
INTRODUCTION
RESULTS
Generation of Bdlaxa Crispr-Cas9 null alleles and of Bdcul4laxa double mutants
BdlaxaCR and BdlaxaTI, and Bdcul4Q127*BdlaxaCR and Bdcul4W203*BdlaxaT381I mutants present similar phenotypes
The loss-of-function of BdCUL4 and BdLAXA affects internode cells elongation
BdCUL4 is required for ligule and represses BdLAXA in auricle formation
BdCUL4 and BdLAXA present antagonistic roles in leaf positioning
BdCUL4 and BdLAXA are required for spikelet architecture and determinacy
BdLAXA is inhibited by BdCUL4 in the control of floral organ number and identity
BdLAXA is required to maintain seed size and roots growth
BdCUL4 and BdLAXA are not necessary for seed abscission
BdCUL4 and BdLAXA regulate secondary cell wall lignification and composition
BdCUL4 and BdLAXA regulate cellulose and lignin associated gene expression
DISCUSSION
MATERIALS AND METHODS
Plant material
Growth conditions
Plasmid construction and transformation of Agrobacterium strains
Callus culture
Transformation of B. distachyon
Genotyping of the transgenic plants and Bdcul4Bdlaxa mutants
qRT-PCR gene expression analysis
Imaging, light microscopy and sample preparation
CHAPTER IV Characterization of potential MtNODULEROOT1 and MtNODULEROOT2 interacting partners participating in nodule and aerial organ development
Abstract
INTRODUCTION
RESULTS
Identification of the M. truncatula ALOG gene
Isolation and characterization of M. truncatula Mtalog1 Tnt1 insertional mutants
Construction and preliminary characterization of the Mtnoot1alog1 and Mtnoot2alog1 double mutants in nodule
Characterization of the Mtalog1 and Mtnoot1alog1 double mutants in aerial development
The MtNOOT1 gene regulate class II MtKNOX gene expression in nodules
DISCUSSION
MATERIALS AND METHODS
Plant material and growth conditions
Transformation of Medicago truncatula
Crossing between noot and Mtalog1 and noot mutant lines and proMtKNOX3::GUS
M. truncatula DNA extraction and Tnt1 insertional mutant genotyping
Construction of the overexpression, MtALOG-GFP and promoter: GUS plasmids
Inoculations of Medicago
Light microscopy and sample preparation
RT-qPCR gene expression analysis
Phylogeny of M. truncatula ALOG genes
GENERAL DISCUSSION
The nbcl1nbcl2 double mutants highlight the role of the NBCL2 genes in the patterning of aerial organs
NBCL genes redundantly control plant architecture
NBCL1 genes regulate shoot branching and control strigolactones production
NBCL genes are involved in flowering-time regulation
NBCL functions in aerial vegetative and reproductive organs patterning are conserved in grasses .
NBCL1-dependent abscission process are not conserved in grass
Investigation of potential interacting partners and downstream targets of NOOT proteins
CONCLUSIONS
REFERENCES
