Inhibition of root hydraulic properties upon drought and salt stress

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Table of contents

General Introduction
1. Drought and salt stress in plant with a central focus on rice
2. Rice root architecture and anatomy
2.1 Rice root architecture
2.2 Rice root anatomy
3. Diversity of AQP isoforms and their substrate specificities in rice
4. Rice AQP expression in normal conditions
4.1 Tissue-specific expression
4.2 Diurnal-specific expression
5. Role of AQPs in the response of rice to drought and salt stress
5.1 Inhibition of root hydraulic properties upon drought and salt stress
5.2 Regulation of AQP expression in rice upon drought and salt stress
5.3 Genetic manipulation of AQPs in rice
6. Context and thesis objectives
References
Chapter I: Sub-cellular markers highlight intracellular dynamics of membrane proteins in response to abiotic treatments in rice
Abstract
Introduction
Results and discussion
Rice transgenic line creation and subcellular localization visualization
Redistribution of PM aquaporins upon salt and drought stress
Dynamic of endocytosis of PM aquaporins upon salt stress
Materials and methods
Molecular cloning of membrane protein markers and plant transformation
Plant materials and growth conditions
Confocal microscopy visualization
ClearSee tissue preparation
Stress application and pharmacological approach
Declarations
Supplementary figures
Supplementary table
References
Chapter II: Genetic manipulations of rice to improve drought and salt tolerance.
Results
Characteristics of transgenic plants compared to wild-type in control condition
Characteristics of transgenic plants compared to wild-type upon drought stress
Characteristics of transgenic plants compared to wild-type upon salt stress
Discussion
Materials and methods
Experimental design
Fraction of transpirable soil water
Leaf water content
Chlorophyll content
Leaf water potential
Stomatal conductance
DAB staining
Na+ and K+ content measurements
Statistical analysis
Acknowledgments
References
Chapter III: Control of water uptake by root system architecture in rice (Oryza sativa L.) 
Abstract
Introduction
Results
Root morphological characteristics
Root hydraulic conductance and conductivity
Effects of azide treatment on Lpr
Effects of China ink treatment on Lpr
Effects of salt stress on the root hydraulics
Covariation of root hydraulic properties and root morphology
Discussion
Root morphological characteristics
Root hydraulic properties
Effects of salt stress on the root hydraulics
Towards a global understanding of root hydraulics strategies in rice
Conclusion
Materials and methods
Plant materials and growth conditions
Root phenotyping
Root hydraulic property measurements
Statistical analysis
Acknowledgments
References