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Table of contents
1 State of the Art
1.1 Introduction
1.2 Mesenchymal stem / stromal cells and their therapeutic benefits
1.3 MSC sources and population heterogeneities
1.4 Expansion process challenges
1.5 Microcarrier-based cultures
1.6 Hydrodynamics potential impact on MSC
1.7 Thesis aims and objectives
2 Improvement of WJ-MSC culture on microcarriers
2.1 Introduction
2.2 Preliminary results
2.3 Development of an automatic cell counting method on microcarriers using image analysis
2.4 Impact of the type of microcarrier in different agitation modes on the expansion performances
2.5 Cell culture on home-made microcarriers
2.6 Chapter conclusions
3 Modelling of microcarriers just-suspended state in a stirred tank bioreactor
3.1 Introduction
3.2 CFD models investigation for simulation of microcarriers suspension in stirred-tank bioreactors
3.3 Dimensional analysis and CFD simulations of microcarrier just-suspended state
3.4 Chapter conclusions
4 Optimization of the impeller design for MSC culture on microcarriers in a stirred tank bioreactor
4.1 Introduction
4.2 Impeller design comparison according to the hydromechanical stress encountered by the microcarriers
4.3 CFD-based strategy to optimize the impeller design adapted to microcarrier suspension in a minibioreactor
4.4 WJ-MSC culture in STR using selected EE impeller designs
4.5 Chapter conclusions
Conclusions
Résumé détaillé de la thèse
Appendices
Appendix A: Supplementary materials and methods
Appendix B: Solid-liquid suspension of microcarriers in stirred tank bioreactor
– Experimental and numerical analysis
Appendix C: Design of experiments of the impeller design optimization
Abstract / Résumé
