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Table of contents
Chapter 1: Ethnobotanical study of plants used in the Tchamba district of Togo to treat infectious diseases
1. Introduction
2. Selection of a suitable area for an ethnobotanical survey: the Tchamba District
3. Recording of traditional knowledge and data collection
3.1. Turning testimonies into data
3.2. CAPITURE of the most relevant plants based on calculated values
4. Results
Description of Pterocarpus erinaceus Poir
Description of Daniellia oliveri (Rolfe) Hutch. et Dalz
5. Discussion
6. Conclusions
Chapter 2: Biological activities of Pterocarpus erinaceus and Daniellia oliveri
1. Introduction
2. Collection and preparation of plant material
3. Extraction and fractionation
3.1. Check-up
3.2. Extraction
3.3. Fractionation
4. Bio-assays
4.1. Antibacterial and antifungal tests
4.2. Anti-cancer assay
4.3. Tests against nematodes
4.4. Tests for cytotoxicity
5. Results
5.1. Antibacterial tests
5.2. Anti-fungal tests
5.3. Anti-cancer tests
5.4. Tests against nematodes
5.5. Cytotoxicity tests
6. Discussion
6.1. Antibacterial and cytotoxicity tests
6.2. Antifungal and cytotoxicity results
6.3. Anti-cancer and cytotoxicity tests
6.4. Nematicidal activities
6.5. Cytotoxicity tests
7. Conclusions
Chapter 3: Analytical studies on the most biologically active extracts of Pterocarpus erinaceus and Daniellia oliveri
1. Introduction
2. Extraction
2.1. Maceration
2.3. Other types of conventional methods that could be easily run in developing countries
2.4. Summarised presentation of some of the modern methods of extraction
3. Clean-up: fractionation
4. Qualitative analysis of the cleaned-up extracts
4.1. Gas chromatography (GC)
4.2. Liquid chromatography (LC)
4.3. Mass spectrometry
4.4. GC-MS on the apolar fractions obtained from the parts of P. erinaceus
4.4.4. GC-MS analysis of the apolar fractions obtained from the parts of D. oliveri
4.5. LC-MS on the polar fractions obtained from the trunk barks of P. erinaceus
5. Purification
5.1. Definition and methods
5.2. Purification performed on the roots of Pterocarpus erinaceus
5.3. Purification performed on the trunk barks of P. erinaceus
6. Qualitative analysis of the isolated compounds
6.1. Nuclear Magnetic Resonance
6.2. Mass spectrometry
6.3. Ultraviolet and infrared absorbance
6.4. Melting point
6.5. Results of the purification process run on the roots of P. erinaceus
6.6. Results of the purification process run on the trunk barks of P. erinaceus
7. Discussion
7.1. Pterocarpus erinaceus
7.2. Daniellia oliveri
8. Conclusions
Chapter 4: Nanoparticles of plant powders
1. Introduction
2. Material and methods
2.1. Plant material
2.2. Biological activity against nematode (Steinernema feltiae), bacterium Escherichia coli) and yeast (Saccharomyces cerevisiae)
3. Results
3.1. Homogenized particles of P. erinaceus barks
3.2. Biological Activity of P. erinaceus barks nanomaterial and respective against E. coli and S. feltiae
3.3. Biological Activity of P. erinaceus barks nanomaterial and respective Extracts against E.
4. Discussion
5. Conclusions




