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Table of contents
Chapter 1 Review of Literature
1 Definition
2 Taxonomy and classification
3 Biochemical composition
3.1 Minerals
3.2 Proteins
3.3 Sugars
3.3.1 Cell wall
3.3.1.1 Green seaweeds
3.3.1.2 Brown seaweeds
3.3.1.3 Red seaweeds
4 Lipids content and fatty acids
5 Applications
5.1 Algae as vegetable in human consumption
5.2 Algae as a source of phycocolloid
5.3 Active ingredients (cosmetics and health)
5.3.1 Caulerpa and Ulva species
5.3.2 Sargassum species
5.3.3 Halymenia species
Chapter 2 Materials and Methods
2.1 Sampling areas and abiotic factors
2.1.1 Overview of the studied location
2.1.2 Samples preparation, drying, and grinding
2.2 Biochemical analyses
2.2.1 Acid and water extraction for the charactrezation of the raw material
2.2.2 Proteins and amino-acids content
2.2.2.1 Amino-acids content
2.2.3 Total sugars content analysis
2.2.4 Uronic acid content analysis
2.2.5 Polyphenol content analysis
2.2.6 Sulfates group content analysis
2.2.7 Ash content analysis
2.2.8 Lipid and fatty acid methyl ester (FAME) analysis
2.2.8.1 Nutritional quality indexes
2.3 Characterization of polysaccharides
2.3.1 Caulerpa racemosa and Ulva lactuca cell wall polysaccharides
2.3.1.1 C. racemosa hot water extraction (HWE)
2.3.1.2 U. lactuca Ulvan extraction
2.3.2 Sargassum polycystum and Sargassum ilicifolium polysaccharides
2.3.3 Halymenia durvillei and Halymenia dilatata polysaccharides
2.3.4 Monosaccharides profile by HPAEC-PAD
2.3.5 Fourier transform infrared (FT-IR) spectroscopy
2.3.6 Minerals by flame atomic absorption spectrophotometry (AAS)
2.4 Analysis of pigments
2.5 Screening of biological activities
2.5.1 Evaluation of cytotoxicity and antiviral activity by cell viability
2.5.2 Antioxidant activity
2.5 Statistical analyses
Chapter 3 Results and Discussions
Results and Discussion
Green Seaweed (Caulerpa racemosa and Ulva lactuca)
3.1 Cell wall polysaccharides purification
3.1.1 First treatment
3.1.2 Polysaccharide extraction
3.1.3 Biochemical composition analysis of extracts
3.1.4 Monosaccharide composition analysis
3.1.5 Fourier Transformed Infra-Red (FT-IR) spectroscopy
3.2 Biological activity
3.2.1 Cytotoxicity and antiviral activity evaluation
3.2.2 Antioxidant activity, DPPH radical scavenging activity
4 Brown Seaweed (Sargassum polycystum and Sargassum ilicifolium)
4.1 Biochemical composition analysis of raw material
4.2 Cell wall polysaccharides purification
4.2.1 First treatment
4.2.2 Polysaccharide extraction
4.2.3 Biochemical composition analysis of extracts
4.2.4 Monosaccharide composition analysis
4.2.5 Fourier Transformed Infra-Red (FT-IR) spectroscopy
4.3 Mineral analysis
4.4 Pigments
4.5 Biological activity
4.5.1 Cytotoxicity and antiviral activity evaluation
4.5.2 Antioxidant activity, DPPH radical scavenging activity
5 Red Seaweed (Halymenia durvillei and Halymenia dilatata)
5.1 Biochemical composition analysis of raw material
5.2 Amino acids composition of raw material
5.3 Lipid composition
5.4 Cell wall polysaccharides purification
5.4.1 First treatment
5.4.2 Polysaccharide extraction
5.4.3 Biochemical composition analysis of extracts
5.4.4 Monosaccharide composition analysis
5.4.5 Fourier Transformed Infra-Red (FT-IR) spectroscopy
5.5 Mineral analysis
5.6 Pigments
5.7 Biological activity
5.7.1 Cytotoxicity and antiviral activity evaluation
5.7.2 Antioxidant activity, DPPH radical scavenging activity
6 Conclusion
References
