Interference with bacterial QS

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Table of contents

List of Figures
List of Tables
List of Abbreviations
Publications & Communications
CHAPTER 1: Introduction
1. The genus Vibrio
1.1 Metabolism
1.2 Classification
1.3 Pathogenic Vibrio species
2. Methods for the identification and quantification
2.1.1 Phenotypical and biochemical identification
2.1.2 Whole genome based methods
2.1.3 Gene sequence based methods
2.1.3.1 Sequencing of housekeeping genes
2.1.3.2 MultiLocus Sequence Analysis or Typing
2.2 Quantification
2.2.1 Culture-based quantification
2.2.2 Fluorescence In Situ Hybridization (FISH)
2.2.3 Real-time Polymerase Chain Reaction (PCR)
3. Ecology of Vibrio
3.1 Abiotic parameters
3.1.1 Temperature
3.1.2 Salinity
3.1.3 Others abiotic parameters
3.2 Biotic parameters
3.2.1 Zooplankton
3.2.2 Phytoplankton
3.3 Population as ecological unit
4. Quorum sensing in Vibrio
4.1 The Aliivibrio fischeri historical model
4.2 Multichannel QS system in Vibrio
4.3 Molecules, biosynthesis and signalling pathways
4.3.1 Autoinducer-1 or N-Acyl-Homoserine-Lactone Quorum sensing
4.3.2 Autoinducer-2 or Furanosyl borate diester Quorum sensing
4.3.3 Cholerae autoinducer-1 Quorum sensing
4.3.4 Other molecules
4.4 QS associated phenotypes
4.5 AHL diversity
4.6 Interactions with other organisms
4.6.1 Interference with bacterial QS
4.6.2 Effects of QS molecules in the interactions with other organisms
5. Objectives of this thesis
6. References
CHAPTER 2: AHL Diversity
Publication 1: Characterization of N-Acyl Homoserine Lactones in Vibrio tasmaniensis LGP32 by a Biosensor-Based UHPLC-HRMS/MS Method
Supplementary Materials
Publication 2: Evidence of a large diversity of N-acyl-homoserine lactones in symbiotic Aliivibrio fischeri strains associated to the squid Euprymna Scolopes
Supplementary Information
CHAPTER 3: Temporal dynamic of AHL production phenotypes
1. Salses-Leucate lagoon
1.1 Characteristics
1.2 Monitoring programs
1.3 Preliminary study
2. References
Publication 3: Genetic Diversity and Phenotypic Plasticity of AHL Mediated Quorum Sensing in Environmental Strains of Vibrio mediterranei
Supplementary Information
CHAPTER 4: Importance of Quorum Sensing in ecological population of Vibrio
Publication 4: Quorum sensing properties of Ecological units of Vibrio
Supplementary Information
CHAPTER 5: Discussion & Perspectives
1. AHL diversity
1.1 Signal specificity: How AHL signalling can be species specific?
1.2 Factors affecting AHL production
How diverse AHL production patterns for a single species can be explained?
2. Prevalence of QS in Vibrio spp
3. In situ QS studies: Complexity of signal production and transmission in the environment
4. Concluding Remarks
5. References