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Table of contents
Table of contents
Table of figures
Table of abreviations
Introduction
I. The ribosome
A. Ribosome and translation
1. Composition of the ribosome
2. Translation initiation
3. Translation elongation
4. Translation termination
B. Ribosome biogenesis
1. Ribosomal RNA transcription
2. Ribosomal RNA processing
3. rRNA modifications and snoRNPs
4. Ribosomal proteins biogenesis
5. Ribosomal protein assembly
6. Pre-ribosomal factors and quality control
7. tRNA biogenesis
II. Ribosome biogenesis as a conductor of cell metabolism
A. Metabolic cues dictate the activity of ribosome synthesis
1. Cell growth and proliferation signals
2. The cell cycle
3. Energy levels
4. Metabolic stress
B. Ribosome biogenesis regulates homeostasis
1. Ribosome biogenesis is monitored at several stages of cell life
2. Ribosome biogenesis drives cellular organization
3. Phenotypic consequences of ribosome biogenesis defects
III. Ribosomes regulate gene expression: the second lives of the housekeepers
A. Ribosome-mediated regulation of translation
1. Ribosomes interact with mRNA cis-regulatory elements
2. Trans-acting factors control translation
3. Ribosome heterogeneity
B. Extra-ribosomal functions of r-proteins
1. When are r-proteins free?
2. r-proteins participate in cell metabolism
3. r-proteins are bound to chromatin
IV. Regulation of ribosome homeostasis by uL11 and Corto
A. The ribosomal protein uL11
1. General features of Drosophila uL11
2. Known functions of uL11 on and off the ribosome
3. Methylation of uL11
B. The transcription factor corto
1. General features of Corto
2. corto participates in epigenetic maintenance of segmental identity during development.
3. corto may be involved in developmental homeostasis
V. Presentation of the thesis project
Results
I. Ribosomal protein uL11 tri-methylated on lysine 3 binds broad genomic regions and displays an exclusion pattern with Corto on chromatin.
A. Results
B. Discussion
C. Materials and methods
II. Editing essential genes: a marker independent CRISPR mutagenesis strategy.
A. Overview
B. Article
C. Complementary data
III. Mutation of a single amino-acid on ribosomal protein uL11 generates a Minute-like phenotype in Drosophila.
A. Results
uL11K3A assembles into functional ribosomes
B. Discussion
C. Materials and methods
Discussion and perspectives
What is the function of uL11K3me3 and CortoCD on chromatin?
Is uL11K3me3 involved in epigenetic regulation of gene expression?
The N-terminal domain of uL11 carries a critical function in Drosophila
The methylation of uL11 lysine 3 is not essential in Drosophila.
Is the function of uL11 lysine 3 methylation strictly transcriptional?
uL11 as an amplifier of ribosome biogenesis?
Bibliography
