Actin cytoskeleton remodelling

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Table of contents

Acronyms
Acknowledgements
INTRODUCTION
CHAPTER I: Biology of CNS development
From the neural crest to the CNS
The glial cells
CHAPTER II: Oligodendrogenesis regulation
DNA binding and chromatin remodelling
Other regulators of oligodendrogenesis
CHAPTER III: Myelination and Remyelination
Myelin generation and function
Oligodendroglial pathologies and therapeutics
CHAPTER IV : Tensins proteins
State of the art
Tensins known functions
Physio-pathology of Tensins dysfunction
Material and methods
Animals and genotyping
Flox Tns3 knockout by tamoxifen injection
Electroporation
MACS
Western Blot
Immunofluorescence
Plasmids and vectors
Primary cells culture
Live cell Imaging
Demyelination Lesions
Objectives and Hypothesis
Results
Article
Detailed results
Tns3 is regulated by keys oligodendroglial factors
Tns3 is highly expressed in an iOL1 subpopulation
Tns3 expression during remyelination
Tns3 constitutive KO induce a sublethal phenotype
Tns3 exon6 frameshift by CRIPSR-Cas9 in NSCs
Optimisation of the Tns3 CRISPR-Cas9 knockout system
Tns3 proteomic expression in oligodendroglia
Deletion of the whole Tns3 locus by CRIPR-Cas9
In vivo Tns3 induced knockout of the postnatal OPCs population
Assessing Tns3-mutant oligodendrocyte defects by video microscopy in neural progenitor cultures
Discussion
Perspectives
References