Retrograde transport machinery

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Table of contents

1. Intracellular transport pathways
1.1 Biosynthetic/secretory pathway
1.2 Endocytic pathways
1.2.1 Late endocytic/degradation pathway
1.2.2 Recycling pathway
1.2.3 Retrograde transport
2. Retrograde transport
2.1 The functions of retrograde transport
2.2 Pathology
2.2.1 Shiga Toxin
2.2.2 Ricin
2.2.3 Cholera toxin
2.2.4 Virus
2.2.5 Other diseases
2.3 Retrograde transport machinery
2.3.1 Clathrin coats
2.3.2 Retromer
2.4 Tools to study retrograde transport
3. Proteomics
3.1 The functions and methods of proteomics
3.1.1 Identification and analysis of proteins
3.1.2 Differential-display proteomics
a). The two-dimensional gel approach
b). Quantitative comparison by mass spectrometry
c). Protein chips
3.1.3 Protein–protein interactions
a). Purification of protein complexes
b). Yeast two-hybrid screening
c). Phage display
3.2 Proteomics in cell biology
3.2.1. A mitochondrial protein complex that links apoptosis and glycolysis
3.2.2. New component in clathrin coated vesicles
3.2.3. Kinase pathways that regulate sex-specific functions in Plasmodium
4. Reaction schemes for proteomics approaches
4.1 SNAP-tag/AGT, a DNA repair enzyme
4.2 Sulfation
4.3 Rapamycin, FK506 and FKBP
4.4 Biotin and streptavidin
4.5 N-hydroxysuccinimide in protein coupling