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Table of contents
LIST OF ABBREVIATIONS
LIST OF FIGURES
LIST OF TABLES
I. IN BRIEF
I. 1. Aim of the study
I. 2. Global context and objectives
I. 3. Summary of results
I. 4. Scientific communication
I. 4. 1. Publications
I. 4. 2. Presentations on international conferences
I. 5. IN BRIEF en Français
II. THEORETICAL BACKGROUND
II. 1. Human red blood cells
II. 2. Membrane transporters of the human RBCs in health
II. 2. 1. Exchangers, pumps, cotransporters
II. 2. 2. Ionic channels
II. 2. 2. 1. Cationic channels
II. 2. 2. 2. Anionic channels
II. 3. Human RBC membrane in disease
II. 3. 1. Infection of the human RBCs by malaria parasite
II. 3. 2. Remodelling of the host erythrocyte membrane Plasmodium falciparum
II. 3. 2. 1. New permeability pathways (NPPs)
II. 3. 2. 2. Morphological changes
II. 4. Electrophysiological studies on anionic channels in human RBCs
II. 5. Molecular nature of anionic channels
II. 6. Physiological role of human red cell membrane channels in health and disease
III. MATERIALS AND METHODS
III. 1. Red blood cells
III. 2. Malaria infected red blood cells
III. 3. Magnetic separation of malaria infected red blood cells
III. 3. 1. Principle
III. 3. 2. Protocol
III. 4. Haemolysis of RESA1 P.falciparum-infected human erythrocytes in isosmotic sorbitol solution
III. 4. 1. Principle
III. 4. 2. Protocol
III. 5. Western blotting of RESA1 P.falciparum-infected human erythrocytes
III. 5. 1. Cells preparation
III. 5. 2. Samples preparation
III. 5. 3. Extraction and denaturation
III. 5. 4. Electrophoresis and blot
III. 6. Immunofluorescence staining and confocal microscopy of RESA1 P.falciparum-infected human erythrocytes
III. 7. Patch-clamp
III. 7. 1. Principle
III. 7. 2. Current recordings
III. 7. 3. Current analysis
III. 8. Percoll-gradient separation of human red blood cells
III. 8. 1. Principle
III. 8. 2. Protocol
IV. RESULTS
IV. 1. First objective: Further clues on electrophysiological characterization of anionic channels in human red cell membrane
IV. 1. 1. Introduction
IV. 1. 2. Results
IV. 1. 3. Discussion
IV. 1. 4. Article
IV. 2. Second objective: The molecular identity and regulation of anionic channels in the physiology and pathophysiology of the human red blood cells
IV. 2. 1. Introduction
IV. 2. 2. Results
IV. 2. 3. Discussion
IV. 2. 4. Article
IV. 3. Third objective: The activation of anionic channels by Plasmodium falciparum and possible involvement of RESA1 protein in this process
IV. 3. 1. Introduction
IV. 3. 1. 1. Ring infected Erythrocyte Surface Antigen (RESA)
IV. 3. 1. 1. 1. Structure
IV. 3. 1. 1. 2. Link with spectrin
IV. 3. 1. 1. 3. Role in malaria infected erythrocyte
IV. 3. 2. Results
IV. 3. 3. Discussion
IV. 4. Forth objective: Physiological role of erythrocyte channels: A unifying hypothesis of senescence, sickle cells and malaria
IV. 4. 1. Introduction
IV. 4. 2. Results
IV. 4. 3. Discussion
V. GENERAL CONCLUSIONS AND PROSPECTS
V. 1. Concluding remarks
V. 2. Discussion and perspectives
REFERENCES
ANNEX
ACKNOWLEDGMENTS
