Scanning electron micrographs of human hair bundles

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Table of contents

I. INTRODUCTION
A. HAIR-CELLS: THE MECHANO-ELECTRICAL TRANSDUCERS
B. THE ARCHITECTURE OF THE ACTIN CORE AND ACTIN DYNAMICS IN THE STEREOCILIA
C. OTHER ACTIN-BASED PROTRUSIONS
D. CONTROL OF STEREOCILIA DIMENSIONS BY MECHANOTRANSDUCTION
II. MATERIALS AND METHODS
A. EXPERIMENTAL PREPARATION OF THE SENSORY TISSUES
B. PHARMACOLOGICAL PERTURBATION OF THE MECHANO-ELECTRICAL TRANSDUCTION MACHINERY
C. MECHANICAL STIMULATION OF HAIR BUNDLES
D. SCANNING ELECTRON MICROSCOPY
E. TRANSMISSION ELECTRON MICROSCOPY
F. IMMUNOLABELLING AND IMMUNOFLUORESCENCE MICROSCOPY
G. INHIBITION OF FORMINS
III. RESULTS
A. MORPHOLOGICAL CHARACTERIZATION OF SACCULAR HAIR BUNDLES OF THE FROG RIVAN
B. EFFECTS OF PHARMACOLOGICAL PERTURBATION OF MECHANO-ELECTRICAL TRANSDUCTION
C. PROBING THE ROLE OF FORMINS FOR ACTIN POLYMERIZATION IN STEREOCILIA
IV. DISCUSSION AND CONCLUSIONS
A. COMPARING RIVAN 92’S TO AMERICAN BULLFROGS’ HAIR CELLS
B. STEREOCILIA WIDENING AND SHORTENING UPON PERTURBATION OF THE MECHANO-ELECTRICAL TRANSDUCTION MACHINERY
V. APPENDIX
A. LATTICE PACKING OF CIRCULAR DISKS
B. SCANNING ELECTRON MICROSCOPY: SAMPLE PREPARATION
C. TRANSMISSION ELECTRON MICROSCOPY