Thin-layer chromatography and scintillation counting

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Table of contents

ABBREVIATIONS
INTRODUCTION
CHAPTER 1. Literature overview
CHAPTER 2 MATERIALS AND METHODS
2.1 Material used in the study
2.2. Pharmacological treatments
2.3. Methods concerning phospholipids analysis
2.3.1 Phospholipid radioactive labeling and lipid extraction
2.3.2. Thin-layer chromatography and scintillation counting
2.4. Methods concerning plant physiology
2.4.1 Histochemical assay
2.4.2. Stomatal assay
2.4.3 ROS visualization and measurement
2.4.4 Callose deposition evaluation
2.4.5 Pathogen assay
2.4.6 Root growth assay
2.5. Methods concerning gene expression analysis
2.5.1 RNA extraction
2.5.2 Transcript abundance evaluation by qPCR
2.5.3 Transcriptome Studies
2.5.4 Statistical Analysis of Microarray Data
2.5.5 In silico analysis of microarray data
2.5.6 Comparison of two sets of transcriptomic data
2.5.7 Data Deposition
2.6 Data analysis and statistics
CHAPTER 3 Involvement of phospholipase D and NADPH-oxidase in salicylic acid signaling cascade
CHAPTER 4 Basal phosphoinositide-dependent phospholipase C activity in Arabidopsis suspension cells as a signalling hub accounting for the overlap in transcriptome remodelling by abscisic or salicylic acid
CHAPTER 5 Phosphatidylinositol-dependent phospholipase C and diacylglycerol kinase are involved in primary responses to flagellin in plant cells
CHAPTER 6 The mutations of both type III phosphatidylinositol-4-phosphate kinases ß1 and ß2 isoforms lead to altered response to auxins and cytokinins in Arabidopsis roots
REFERENCES