The film hydration method

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Table of contents

Introduction
1. Sensitivity of cells to uneven distributions of peptides and proteins
1.1. Representative proteins of interest
1.2. Characteristic scales: concentrations, time and lengths
1.3. Summary and conclusion
I. Light-controlled release, a review
2. Light-sensitive liposomes
2.1. Light-induced increase of permeability
2.2. Light-triggered pore formation
2.3. Membrane disruption
2.4. Encapsulation yield and preparation conditions
2.5. Summary on light-sensitive vesicles
3. Micro- and nanometer-sized hydrogels
3.1. Photodegradable hydrogels
3.2. Light-triggered gel collapse
3.3. Encapsulation yield and loading constraints
4. Aqueous core—polymer shell capsules
4.1. Layer-by-layer assemblies
4.2. Polymersomes
4.3. Capsules made of solid polymers
4.4. Hybrid membranes
4.5. Role of the stimulation wavelength
Summary
II. Light-sensitive liposomes
5. Surfactant sensitized vesicles
Introduction
5.1. Liposome solubilization by the azoTAB surfactant
Conclusion
6. Azobenzene-based polymer sensitizer
Introduction
6.1. Experimental conditions
6.2. Interpretation of typical photo-triggered release patterns
6.3. Data analysis
Conclusion
III. Temperature-sensitive capsules made by the reverse emulsion method
Introduction
7. PolyNIPAM-based millimeter sized capsules
7.1. Adaptation of published protocols
7.2. PolyNIPAM-based capsules
7.3. Effect of mixed membrane composition
8. PolyNIPAM-based nanocapsules
8.1. Description of the synthesis of nanometer sized capsules
8.2. PolyNIPAM nanocapsules characterization
9. UCST polymer-based millimeter-sized capsules
9.1. Characterization of the UCST polymer
9.2. Adaptation of the capsule preparation procedure to the UCST polymers .
9.3. Characterization of capsules made from UCST polymers and ovalbumin
Conclusions & perspectives
Liposomes
Polymer capsules
IV. Appendices
A. Role of molecular gradients on biological systems
B. Currently used gradient generators
C. Materials & Methods
D. Experimental techniques
E. Preparation and characterization of liposomes
F. Preparation and characterization of polymer capsules
G. Quantitative and real-time FRAP measurements
Bibliography