Cell Junctions and Adhesions

For more info about our services contact : help@bestpfe.com

Table of contents

1 French abstract 
2 Introduction 
2.1 Forward
2.2 Epithelial cells and their functions
2.2.1 Single cell architecture: the cytoskeleton
2.2.2 Cell Junctions and Adhesions
2.2.3 Cell Polarity
2.2.4 Multicellular architecture
2.3 In vitro models of epithelia
2.3.1 Collective cell migration, tissue dynamics, ngers and complex ow patterns
2.3.2 Actin cables during re-epithelialization
2.3.3 Population connement
2.3.4 Limitations on 2D models
2.4 Assembling epithelial tissues in vivo: tubulogenesis
2.4.1 Tube formation
2.4.2 Tube elongation
2.4.3 Tube diameter regulation
2.5 Two case studies of in vivo curvature
2.5.1 Drosophila trachea
2.5.2 Drosophila egg chamber
2.6 In vitro studies of curvature
2.6.1 Denition of terms: curvature
2.6.2 Two dimensional in-plane curvature assays
2.6.3 Three dimensional curvature assays
2.6.4 Out-of-plane curvature assays
3 Materials and Methods 
3.1 Surface fabrication
3.1.1 Pillar assays
3.1.2 Glass wires
3.1.3 Polystyrene (PS) wires
3.2 Surface coating
3.2.1 Fibronectin
3.2.2 Pll-Peg
3.2.3 Micropatterning
3.3 Cell culture
3.3.1 Cell lines
3.3.2 Culture protocols
3.4 Microscopy
3.4.1 Video microscopy
3.4.2 Confocal spinning disk microscopy
3.4.3 Two photon laser ablation
3.5 Immuno uorescence
3.6 Drug Inhibitions
3.7 Image processing
3.7.1 Image projections
3.7.2 Image feature orientation
4 Results & Discussion 
4.1 Pillar vs. wire assays
4.2 Static properties of the monolayer
4.2.1 Polarity
4.2.2 Cell morphology
4.2.3 Density proles
4.3 Curvature-induced EMT
4.3.1 Connement vs. curvature
4.4 Monolayer molecular architecture
4.4.1 Actin cytoskeleton alignment
4.4.2 Photoablation of stress bers and cables
4.5 Collective migration
4.5.1 Front velocity vs. radius
4.5.2 Connement vs. curvature
4.5.3 Mechanisms governing collective migration
4.5.4 Theoretical models of migration
4.6 Extreme curvatures: cone tips
5 Conclusion