Bacterial identification by 16S rRNA gene sequencing

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Table of contents

ACKNOWLEDGMENTS
TABLE OF CONTENTS
GLOSSARY
GENERAL INTRODUCTION
HOLOBIONTS IN MULTICELLULAR EUKARYOTES – CHALLENGES & KEY QUESTIONS
HOLOBIONTS IN TERRESTRIAL MODELS
HOLOBIONTS IN BROWN MACROALGAE – A UNIQUE GROUP OF MULTICELLULAR EUKARYOTES
FACTORS THAT SHAPE THE ALGAL HOLOBIONT
MACROALGAL HOLOBIONTS IN A CHANGING ENVIRONMENT
THESIS SUBJECT: THE RESPONSE OF ECTOCARPUS HOLOBIONTS TO CHANGING SALINITY
CHAPTER 1. EXPLORING THE CULTIVABLE ECTOCARPUS MICROBIOME
ABSTRACT
INTRODUCTION
MATERIAL AND METHODS
RESULTS
DISCUSSION
CONFLICT OF INTEREST
AUTHOR CONTRIBUTIONS
FUNDING
ACKNOWLEDGMENTS
CHAPTER 2. CULTIVABLE BACTERIA FROM THE ECTOCARPUS SURFACE – APPLICATIONS
INTRODUCTION
I. THE IMPACT OF CULTIVABLE BACTERIAL SYMBIONTS ON THE FRESHWATER RESPONSE IN ECTOCARPUS SUBULATUS FRESHWATER STRAIN
II. SPECIFICITY OF CROSS-LINEAGE CROSS-TALK: DO ECTOCARPUS-DERIVED BACTERIA INTERACT WITH ULVA GAMETES?
III. METABOLIC COMPLEMENTARITY BETWEEN ECTOCARPUS AND ASSOCIATED CULTIVABLE BACTERIA: EXPERIMENTAL VERIFICATION OF IN SILICO PREDICTED BENEFICIAL COMMUNITIES
CHAPTER 3. OMICS APPROACHES TO EXPLORE THE ROLE OF THE ECTOCARPUS MICROBIOME DURING
ACCLIMATION TO FRESH WATER
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
FINAL CONCLUSIONS & PERSPECTIVES
BIBLIOGRAPHY
ANNEX 1 SUPPLEMENTARY DATA
SUPPLEMENTARY DATA CHAPTER 1
SUPPLEMENTARY DATA CHAPTER 2
SUPPLEMENTARY DATA CHAPTER 3
ANNEX 2 THE VISUALIZATION AND LOCALIZATION OF BACTERIA ON THE SURFACE OF ECTOCARPUS
SUBULATUS FWS USING FISH AND SEM TECHNIQUES.
INTRODUCTION
ANNEX 3 THE GENOME OF ECTOCARPUS SUBULATUS HIGHLIGHTS UNIQUE MECHANISMS FOR STRESS
TOLERANCE IN BROWN ALGAE
ANNEX 4 ATTENDED CONFERENCES & MEETINGS
ANNEX 5 ATTENDED COURSES
LIST OF FIGURES
LIST OF TABLES