Chemical constraints resulting from cryoconcentration and water loss

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Table of contents

FOREWORD
BIBLIOGRAPHIC REVIEW
I. Freezing lactic acid bacteria
II. Improvement of the cryoresistance of LAB
III. FTIR spectroscopy: a powerful vibrational approach to study LAB
EXPERIMENTAL APPROACH
RESULTS & DISCUSSION
I. Comparative cryoresistance and genomic analyses of three strains of Lactobacillus delbrueckii subsp. bulgaricus
II. Biophysical characterisation of the Lactobacillus delbrueckii subsp. bulgaricus membrane during cold and osmotic stress and its relevance for cryopreservation
III. Subcellular membrane fluidity of Lactobacillus delbrueckii subsp. bulgaricus under cold and osmotic stress
IV. Towards the analysis of single bacteria in an aqueous environment using synchrotron infrared micro-spectroscopy
CONCLUSIONS & FUTURE PERSPECTIVES
REFERENCES
APPENDICES
I. Preparation of bacterial inocula
II. Supplementary data to Chapter I of Results & Discussion
III. Supplementary data to Chapter II of Results & Discussion
IV. Résumé substantiel de la thèse en français