Confirmation of differential expression of selected genes by real time quantitative RT-PCR

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Table of contents

CHAPTER 1 Introduction
Melanocytes
Melanoma
Melanoma susceptibility and altered signalling
Conventional anti-melanoma therapy
Anti-melanoma immunotherapy
Gene expression profiling in human melanoma
Spontaneous melanoma regression
Immune escape mechanisms
Animal models of melanoma
Pigs in biodmedical research
Melanoblastoma bearing Libechov Minipig (MeLiM)
Spontaneous melanoma regression in MeLiM
CHAPTER 2 Introduction of: “Identification of differentially expressed genes in spontaneously regressing melanoma using the MeLiM Swine Model”
Comment on Suppression Subtractive Hybridization (SSH) method
Paper I: Identification of differentially expressed genes in spontaneously regressing melanoma using the MeLiM Swine Model
CHAPTER 3 Introduction of: “Gene Expression Signature for Spontaneous Cancer Regression in Melanoma Pigs”
Comment on microarray processing method
Target Production and Hybridization
Reannotation of porcine Affymetrix Probesets
Paper II: Gene Expression Signature for Spontaneous Cancer Regression in Melanoma Pigs 79 Phenotypic characterization of tumor immune infiltrate
1. Characterization of highly pigmented cells
2. Phenotypic characterization of Tumor Infiltrating Lymphocytes (TIL)
CHAPTER 4 General Discussion
Differentially expressed genes between regressive and progressive melanoma
Time dependent gene expression profiling during melanoma progression and regression
Comparison of obtained transcriptomic results
Hypothesis of regression mechanism in MeLiM
Expression profiles and other regressing malignancies
Perspectives
REFERENCES