Cryptic diversity and ecophenotypy in planktonic foraminifera

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Table of contents

Chapitre 1 : Protocole d’extraction ADN
Abstract
1. Introduction
1.1. Excellence and caveats of pelagic fossil records
1.2. Morphological versus genetic species concepts in planktonic foraminifera
1.3. The value of direct morpho-genetic comparisons
1.4. Orbulina universa d’Orbigny
2. Material and methods
2.1. Sample collection
2.2. DNA extraction
2.3. Molecular analyses
2.4. Shell preservation
3. Results
3.1. Efficacy of the GITC
3.2. GITC and shell preservation
3.3. Identification of genotypes
3.4. Distribution of genotypes and hydrography
4. Discussion
4.1. Advantages of the GITC
4.2. Form and function in pelagic taxa
5. Conclusion
Acknowledgments
References
Chapitre 2 : Diversité morphologique des espèces cryptiques d’Orbulina universa (d’Orbigny)
Abstract
1. Introduction
1.1. Morphological species and cryptic diversity in planktonic foraminifera
1.1. Orbulina universa d’Orbigny, 1839
2. Material
3. Methods
3.1. DNA extraction, amplification and RFLP analysis
3.2. Biometry
3.2. Statistical analysis
4. Results
4.1. Identification of genotypes
4.2. Geographic distribution of genotypes and hydrography
4.3. Biometry
5. Discussion
5.1. Environmental significance of shell porosity in Orbulina universa
5.2. Cryptic diversity and ecophenotypy in planktonic foraminifera
5.3. Implications for paleoceanography
6. Conclusion
Acknowledgments
References
Chapitre 3 : Diversité morphologique des espèces cryptiques de Truncorotalia truncatulinoides d’Orbigny
Abstract
1. Introduction
2. Material
3. Methods
3.1. DNA extraction, PCR amplification and RFLP analysis
3.2. Shape Descriptors
3.3. Shape Analyses
4. Results
4.1. Identification of genotypes
4.2. Geographic distribution of genotypes
4.3. Morphological differentiation among cryptic species
4.4. A model for morphological recognition of cryptic species
5. Discussion
6. Conclusion
Acknowledgments
References
Chapitre 4 : Diversité cryptique de Globoconella inflata
Abstract
1. Introduction
2. Results
2.1. Genetic Variation in Globoconella inflata
2.2. Geographic Distribution of Genotypes
2.3. Inter-Hemisphere comparative distribution of G. inflata in surface sediments
2.4. Biometry
3. Discussion
3.1. ITS for Identifying Genetic Variability in Planktonic Foraminifera
3.2. Cryptic Diversity as a Tool for Monitoring Past Migrations of the Antarctic Subpolar Front
4. Material and Methods
4.1. Sample Collection
4.2. DNA Extraction, Amplification and Sequencing
4.3 DataSets, Alignement and DNA Sequence Analysis
4.4. RFLP Analysis.
4.5. Spatial and Environmental Distribution of Globoconella inflata
4.6. Biometrics
Acknowledgments
References
Chapitre 5 : Impact de la diversité cryptique sur les reconstitutions paléocéanographiques
Abstract
1. Introduction
2. Material
2.1. Sample collection
2.2. Core-top and environmental dataset
3. Methods
3.1. Molecular analysis
3.2. Theoretical distribution of genotypes
3.3. Training sets
3.4. Transfer functions
3.5. Temperature estimation robustness
4. Results
4.1. Phylogenetic analysis of Globigerina bulloides
4.2. RFLP identification of genotypes
4.3. Distribution of genotypes
4.4. Modeled abundances
4.5. Improvement of assemblage-based SST reconstructions
4.6. Robustness
5. Discussion
5.1. Improvement of the transfer function
5.2. Contribution of the cryptic species
6. Conclusion
Acknoledgments
References
Conclusions
Bibliographie

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