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Table of contents
Chapter 1 – State of the art
1 Harmful algal blooms
1.1 Phytoplankton
1.2 Harmful algal blooms
1.3 Increase of HABs events
1.4 Factors structuring the dynamic of phytoplankton and HABs
2 Allelochemical interactions
2.1 Chemical ecology
2.2 Definition of allelopathy
2.3 Allelochemical interactions in marine environment
2.4 Ecological significance
2.5 Environmental control of allelochemical interactions
2.6 Microalgae producing allelochemicals
3 The genus Alexandrium
3.1 Classification
3.2 Life cycle
3.3 Biology
3.4 Toxin production
3.5 Alexandrium spp. worldwide geographical distribution
3.6 Alexandrium spp. outbreaks in Australia
3.7 Alexandrium spp. outbreaks in France
3.8 Environmental control of Alexandrium spp. blooms
4 Alexandrium spp. allelochemical potency
4.1 Allelochemical potency within the genus Alexandrium
4.2 Effects of allelochemicals on protists and microbial communities
4.3 Allelochemicals mode of action
4.4 Effects of allelochemicals on biological membranes
4.5 Environmental regulation of Alexandrium spp. allelochemical potency
4.6 Chemical characterization of Alexandrium spp. allelochemicals
4.7 Alexandrium spp. exudates
5 Biological activities of Alexandrium spp. exudates
5.1 Hemolytic and cytotoxic activities
5.2 Toxicity to bivalves
5.3 Toxicity to fish
6 Chemical nature of Alexandrium spp. exudates
6.1 Characterization of Alexandrium spp. exudates
6.2 Characterization of hemolytic and cytotoxic compounds
6.3 Characterization of ichtyotoxic compounds
Objectives and organization of the thesis
Chapter 2 : Unraveling the mechanisms of allelochemical interactions
Article 1 – Allelochemicals from Alexandrium minutum induce rapid inhibition of metabolism and modify the membranes from Chaetoceros muelleri
Article 2 – Allelochemical interactions between Alexandrium minutum and Chaetoceros muelleri: insights into photosynthesis and membrane integrity
Chapter 3 : Development of a bioassay to quantify allelochemical interactions from Alexandrium minutum
Article 3 – A rapid quantitative fluorescence-based bioassay to study allelochemical
interactions from Alexandrium minutum
Chapter 4 : Effects of abiotic factors on allelochemical potency
Article 4 – A multi-trait approach reveals the effects of Cu on the physiology of an allelochemical-producing strain of Alexandrium minutum
Chapter 5 : What is the chemical nature of Alexandrium minutum allelochemicals ?
Chapter 6 – General discussion
1 Chemical nature of allelochemicals
2 Allelochemicals mode of action
3 Interactions between allelochemicals and cytoplasmic membranes
4 Interactions between allelochemicals and photosynthetic membranes
5 Understanding the mode of action to explain the variability in the sensitivity to allelochemicals
6 Variability of allelochemical potency from Alexandrium strains
7 The relevance of laboratory studies in an environmental context
8 Beyond allelochemical interactions
Conclusions and perspectives
PhD Outcomes
References (Introduction and General discussion)



