Freeze-drying of porous gelatin in PEGDA lattices

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Table of contents

Outline
Chapter 1 Introduction
1.1 Cell and cellular environment
1.1.1 Cell
1.1.2 Cell microenvironment
1.1.3 Cell culture
1.2 Stem cells and induced luripotent stem cells
1.2.1 Stem cells
1.2.2 Neural progenitor cells
1.2.3 Induced pluripotent stem cells
1.3 Bioscaffolds for tissue engineering
1.3.1 Biomaterials
1.3.2 Scaf folds requirements and fabrication methods
1.3.3 Key factors of scaffold affecting cell performance
1.3.4 Scaffolds for tissue engineering applications
1.4 Research objectives
Reference
Chapter 2 Fabrication methods
2.1 UV lithography
2.1.1 Mask preparation
2.1.2 Substrate cleaning
2.1.3 Photoresist processing
2.2 Soft lithography
2.3 Electrospinning
2.3.1 Principle and parameters
2.3.2 Materials
2.3.3 Applications
2.3.4 Limitations
2.4 3D pri nting
2.4.1 Advantages
2.4.2 Printing technologies
2.4.3 Materials
2.4.4 Applications
2.4.5 Limitations
2.5 Freeze drying
2.5.1 Principle
2.5.2 Applic
2.5.2 Applicationsations
2.6 SelfSelf–organization of polymer scaffoldsorganization of polymer scaffolds
2.7 Parylene C depositionParylene C deposition
2.7.1 Introduction of parylene C
2.7.2 Principle
2.7.3 Working process
2.7.4 Substrates for Parylene C coating
2.7.5 Applications
Reference
Chapter 3 Fabrication of hierarchic scaffolds by 3D printing and freeze–drying for drying for cell culture and neuron differentiationcell culture and neuron differentiation
3.1 Introduction
3.2 FabricatFabrication of hierarchic scaffoldsion of hierarchic scaffolds
3.2.1 3D printing of honeycomb lattice made of PEGDA3D printing of honeycomb lattice made of PEGDA
3.2.2 Freeze–drying of porous gelatin in PEGDA latticesdrying of porous gelatin in PEGDA lattices
3.3 Cell culture studiesCell culture studies
3.4 3D printed PEGDA/porous gelatin scaffold for neuronal 3D printed PEGDA/porous gelatin scaffold for neuronal differentiationdifferentiation
3.4.1 Fabrication of 3D printed PEGDA/porous gelatin scaffold
3.4.2 Culture and differentiation of neural progenitor cells
3.5 ConclusionConclusion
Reference
Chapter 4 Fabrication of self–organized porous PCL membrane for improved cell organized porous PCL membrane for improved cell culture and hiPSCs differentiationculture and hiPSCs differ
4.1 Introduction
4.2 Fabrication of the culture patchation of the culture patch
4.2.1 Fabrication of PEGDA honeycomb frame
4.2.2 Self–organization of porous PCL membraneorganization of porous PCL membrane
4.3 Cell based assays
4.4.1 Cell proliferation on 2D porous PCL patch
4.4.2 Cellular uptake
4.4.3 Gene transfectiontransfection
4.5 Cardiac differentiation of hiPSCs on 2D porous PCL
4.5.1 HiPSCs culture on 2D porous PCL
4.5.2 Pluripotency of hiPSCsency of hiPSCs
4.5.3 Proliferation of hiPSCs
4.6 Biodegradation
4.7 Conclusionlusion
Reference
Chapter 5 Monolayer gelatin nanofibers on PDMS frame for cardiac differentiationdifferentiation
5.1 Introductionon
5.2 Fabrication of PDMS/gelatin nanofibers patch
5.2.1 Fabrication of PDMS honeycomb frame
5.2.2 Electrospinning of gelatin nanofibersgelatin nanofibers
5.3 Cell based assays
5.3.1 Culture and seeding of hiPSCs
5.3.2 Cell viability assay
5.3.2 Cell viability assay
5.3.3 Pluripotency of hiPSCs
5.3.4 Cardiac differentiation
5.3.5 Immunostaining
5.3.6 Calcium imaging
5.3.7 Electric stimulationElectric stimulation
5.3.8 Drug test
5.3.9 Deformation of PDMS frame
5.4 Conclusion
Reference
Chapter 6 Conclusion and perspective
Reference
Appendix A Patterned parylene C for cell adhesion, spreading and alignment ned parylene C for cell adhesion, spreading and alignment studiesstudies
A.1 Introduction
A.2 Parylene C pattern fabrication
A.2.1 Fabrication process2.1 Fabrication process
A.2.2 SEM observation
A.3 Cell culture studiesll culture studies
A.3.1 Gelatin coating on parylene C film
A.3.2 Cell adhesion and spreading
A.3.3 Cell alignment
A.4 Conclusion
Reference
Appendix B Porous gelatin patch for VEGF loading and controlled release
B.1 Introduction
B.2 Fabrication of VEGF loaded porous gelatin patch
B.2.1 Fabrication of porous gelatin patch
B.2.2 VEGF loading on porous gelatin patch
B.3 Results
B.3.1 Porous gelatin patch
B.3.2 VEGF loading and release
B.4 Conclusion
Reference
Appendix C French summary