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Table of contents
I. IN BRIEF
I. 1. Aims
I. 2. Summary of results
I. 3. Scientific communication
I. 3. 1. Publications
I. 3. 2. Oral communications
I. 4. IN BRIEF en Fraçais
I. 5. IN BRIEF po polsku
II. GENERAL CONTEXT
II. 1. Oxygen
II. 2. Haemoglobin
II. 3. Erythrocytes
II. 4. Human red blood cell
II. 5. RBC membrane transporters
II. 5. 1. Pumps, exchangers, cotransporters
II. 5 .2. Aquaporins
II. 5. 3. Ionic channels
II. 5. 3. 1. Cationic channels
II. 5. 3. 2. Anionic channels
II. 5. 3. 3. Physiological role of ionic channels
III. MATERIALS AND METHODS
III. 1. Contribution of ionic channels in dehydration-elicited rehydration: lysis curves and osmotic fragility data acquisition
III. 1. 1. Experimental design
III. 1. 2. Solutions
III. 1. 3. Preparation of red blood cells
III. 1. 4. The biphasic OFC protocol
III. 1. 5. Measurement of the volume-fraction of RBC cytosol lost by Ca2+-induced exovesiculation
III. 1. 5. 1. Processing of vesicle samples for transmission and scanning electronmicroscopy
III. 2. Electrophysiology
III. 2. 1. Preparation of cells
III. 2. 2. Solutions
III. 2. 3. Current recordings
III. 3. Chemicals
III. 4. Red blood cell model
IV. RESULTS
IV. 1. First question: Are RBC’s membrane channels involved in rehydration elicited by isosmotic dehydration?
IV. 1. 1. Results
IV. 1. 2. Discussion
IV. 2. Second question: Does membrane deformation induce channel activity?
IV. 2. 1. Results
IV. 2. 1. 1. Evidence for spontaneous channel activity following seal formation
IV. 2. 1. 2. Transient nature of recorded currents
IV. 2. 1. 3. Identification of Gardos channels
IV. 2. 1. 4. Simulation by mathematical RBC model
IV. 2. 1. 5. Evidence for anionic channel activation
IV. 2. 2. Discussion
IV. 2. 2. 1. Activation of Gardos channels
IV. 2. 2. 2. Activation of anionic channels
IV. 2. 2. 3. Transient nature of Gardos channel activity
IV. 2. 2. 4. Fast transitions
IV. 3. Third question: What is the molecular identity of anionic channels present in RBC membrane?
V. GENERAL CONCLUTIONS AND PROSPECTS
ANNEX #1
ANNEX #2
ANNEX #3
ANNEX #4
ANNEX #5
REFERENCES
