Hyperaemic response paradigm

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Table of contents

ABSTRACT
RESUMO
RESUME
GENERAL INTRODUCTION
LIST OF PUBLICATIONS
CHAPTER 1. BIBLIOGRAPHIC REVIEW: NON-INVASIVE STUDY OF GENETIC MUSCLE DISORDERS
MUSCULAR DYSTROPHIES
Vascular alterations in Duchenne muscle dystrophy
CONGENITAL MYOPATHIES
ANIMAL MODELS FOR GENETIC MUSCLE DISEASES
Animal models for Muscular Dystrophies
Animal models for congenital myopathies
NON-INVASIVE EVALUATION OF GENETIC MUSCLE DISEASES
NMR IN THE STUDY OF ANIMAL MODELS FOR GENETIC MUSCLE DISEASES
CHAPTER 2. QUANTITATIVE T2 COMBINED WITH TEXTURE ANALYSIS OF NUCLEAR MAGNETIC RESONANCE IMAGES IDENTIFY DIFFERENT DEGREES OF MUSCLE INVOLVEMENT IN THREE MOUSE MODELS OF MUSCLE DYSTROPHY: MDX, LARGEMYD AND MDX/LARGEMYD
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Ethics Statement
Animals
Magnetic Resonance Imaging acquisition and analysis
Histological analysis
Statistic analysis
RESULTS
Muscle T2
Muscle texture analysis
Histological analysis
DISCUSSION
CONCLUSIONS
SUPPORTING INFORMATION
S2.1. T2 calculation from two images at different echo times
S2.2. Features selected for Texture Analysis
COMPLEMENTS TO THE MANUSCRIPT
Validation of the 2 points T2 measurements with a multiecho sequence
Post-mortem changes in the T2 values
CHAPTER 3. STRUCTURAL AND FUNCTIONAL ALTERATIONS OF SKELETAL MUSCLE MICROVASCULAR NETWORK IN DYSTROPHIN-DEFICIENT MDX MICE
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Mice
Microvascular network organisation in three dimensions
Histology
Morphometric analysis
Nuclear Magnetic Resonance analysis
Statistics
RESULTS
Normal microvascular network organisation in young-adult Flk1GFP/+::mdx mouse
Muscle blood perfusion is modified in young-adult mdx mice.
Muscle bioenergetics in young-adult mice (Table 3.1)
Alteration of microvascular network organisation in old Flk1GFP/+::mdx mouse
Alteration of muscle perfusion in old mdx mice
Muscle bioenergetics in 12 month-old mice (Table 3.2)
DISCUSSION
CONCLUSION
SUPPLEMENTARY INFORMATION
S3.1. Nuclear Magnetic Resonance analysis
CHAPTER 4. NON-INVASIVE NMR STUDY OF THE MOUSE MODEL FOR CENTRONUCLEAR MYOPATHY WITH MUTATION IN THE DYNAMIN-2 GENE
INTRODUCTION
MATERIALS AND METHODS
Animals
Nuclear Magnetic Resonance (NMR)
Data analysis
Histological analysis
Statistical analysis
RESULTS
Morphometrical evaluation
T1 measurements
T2 measurements
Histological analysis
DISCUSSION
CHAPTER 5. PILOT FUNCTIONAL AND METABOLIC EVALUATION OF THE KI-DNM2R465W MICE
INTRODUCTION
PILOT STUDY 1. EXERCISE AS THE PARADIGM OF MUSCLE STRESS IN KI-DNM2R465W MICE
Materials and methods
Results
Discussion
PILOT STUDY 2. PROLONGED ISCHEMIA AS THE PARADIGM OF MUSCLE STRESS
Materials and Methods
Results
Discusssion
PILOT STUDY 3. REGENERATION IN THE DNM2 MICE: T1, T2 AND FUNCTIONAL ANALYSIS AFTER INJURY
Materials and Methods
Results
Discussion
CHAPTER 6. EVALUATION OF THE POTENTIAL USE OF MICRO-COMPUTED TOMOGRAPHY IN THE STUDY OF MUSCLES FROM MURINE MODELS FOR MUSCLE DYSTROPHIES
INTRODUCTION
MATERIALS AND METHODS
Animals
Muscle injury with electroporation
Micro-CT
Data Analysis
Statistical Analysis
RESULTS
Phenotypical characterization of the dystrophic muscle with micro-CT
Evaluation of injured muscle with micro-CT
DISCUSSION
GENERAL CONCLUSIONS AND PERSPECTIVES
BIBLIOGRAPHY