(Downloads - 0)
For more info about our services contact : help@bestpfe.com
Table of contents
CHAPTER 1. INTRODUCTION
1.1. CONTEXT OF THE STUDY
1.2. BACKGROUND
1.2.1. Yarrowia lipolytica
1.2.2. Metabolic engineering tools for Y. lipolytica
1.2.3. The biotechnological applications of Y. lipolytica
1.2.4. Lipid production in Y. lipolytica
1.2.5. Odd-chain fatty acids (OCFAs)
1.3. OBJECTIVES
CHAPTER 2. MATERIALS AND METHODS
2.1. STRAINS, MEDIA, AND GROWTH CONDITIONS
2.2. CLONING
2.2.1. General molecular biology
2.2.2. Gene synthesis
2.2.3. Plasmid construction
2.3. CONSTRUCTION OF Y. lipolytica STRAIN
2.3.1. Transformation
2.3.2. Verification of construction in Y. lipolytica
2.3.3. Re-use of marker
2.4. ANALYSIS
2.4.1. Growth
2.4.2. Fluorescence
2.4.3. Metabolites
2.4.4. Lipids
2.4.5. Morphology
2.4.6. Gene and protein sequence
CHAPTER 3. DEVELOPMENT OF SYNTHETIC BIOLOGICAL TOOLS -SYNTHETIC INDUCIBLE PROMOTER
3.1. INTRODUCTION
3.2. ENGINEERING OF ERYTHRITOL-INDUCIBLE PROMOTERS
3.3. APPLICATION OF THE SYNTHETIC INDUCIBLE PROMOTER FOR RECOMBINANT PROTEIN PRODUCTION; THE EXPRESSION OF CALB
3.4. DISCUSSION
CHAPTER 4. PRODUCTION OF ODD-CHAIN FATTY ACIDS (OCFAS)
4.1. IMPROVE PROPIONATE TOLERANCE
4.1.1. Introduction
4.1.2. Results
4.1.3. Discussion
4.2. INCREASE THE PRODUCTION OF OCFAS
4.2.1. Introduction
4.2.2. Results and discussion
CHAPTER 5. ENGINEERING PRECURSOR POOLS FOR INCREASING LIPID PRODUCTION
5.1. INTRODUCTION
5.2. INCREASING MALONYL-COA POOL
5.3. INCREASING ACETYL-COA POOL
5.4. INCREASING PROPIONYL-COA POOL FOR THE OCFA PRODUCTION
5.5. DISCUSSION
CHAPTER 6. DE NOVO PRODUCTION OF OCFAS FROM GLUCOSE
6.1. INTRODUCTION
6.2. RESULTS
6.3. DISCUSSION
CHAPTER 7. CONCLUSION AND PERSPECTIVES
