Microtubule associated proteins

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Table of contents

Introduction
1. The cell cycle
1.1. Interphase
1.2. Mitosis
2. The microtubule cytoskeleton
2.1. Microtubule structure
2.2. Microtubule dynamics
2.3. Microtubule Associated Proteins
2.3.1. Stabilizing MAPs
2.3.1.1. Plus-­‐end trancking proteins (+TIPs)
2.3.1.2. Hurp
2.3.2. Destabilizing MAPs
2.3.3. Motor proteins
2.3.3.1. Dyneins
2.3.3.2 Kinesins
2.4. Microtubules during the cell cycle
2.4.1. Microtubules in interphase
2.4.2. Microtubules in mitosis
2.4.2.1. Pathways of microtubule spindle assembly
2.4.2.1.1. Centrosome pathway
2.4.2.1.2. Chromosome pathway
2.4.2.1.3. Augmin pathway
2.4.3. Microtubules in anaphase
2.4.4. Microtubules in cytokinesis
2.5. Centers of microtubule organization
2.5.1. The centrosome
2.5.2. Non-­‐centralized microtubule organization
3. Regulation of centrosome-­‐linked activities
3.1. Centrosome cycle
3.2. PCM formation
3.3. Regulation of centrosome cycle
3.3.1. License to duplicate and cell cycle control of centriole duplication
3.4. Centrosome cycle of genome stability
3.5. PLK4 as a master regulator of centriole duplication
3.5.1. PLK4 structure
3.5.2. PLK4 regulation
3.4.3. PLK4 localization to the centrosome and function in centriole assembly
3.4.4. PLK4 role in centriole over-­‐duplication and de novo formation
3.4.5. PLK4 activity in acentriolar cells
4. Mammalian female meiosis in the absence of centrioles
4.1. Disapearance of centrioles
4.2. Protracted prophase I arrest
4.2.1. Control of prophase I arrest by cAMP
4.2.2. Regulation of Cyclin B levels by APC/C
4.2.3 Governing the microtubules in prophase I in the absence o centriole
4.3. Meiotic maturation
4.3.1. NEBD
4.3.2. Acentriolar assembly of the first meiotic spindle
4.3.3. Spindle bipolarization and MTOC sorting to the poles
4.3.4. Chromosome congression and k-­‐fibers assembly
4.3.5. The SAC in female meiosis
4.3.6. Spindle migration to the cortex and chromosome segregation
4.3.7. Polar body extrusion and metaphase
II arrest
Results
1. Rebuilding MTOCs upon centriole loss during mouse oogenesis
2. Plk4 regulation of acentriolar MTOC assembly is critical for meiosis in the mouse oocyte
Discussion
Towards understanding of PCM assembly Regulation of PCM recruitment to acentriolar MTOCs
Centriole elimination in the mouse oocyte