Ocean acidification and carbon-concentrating mechanisms

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Table of contents

TABLE OF CONTENTS
LIST OF FIGURES
LIST OF PERSONAL PUBLICATIONS AND COMMUNICATIONS
LIST OF ABBREVIATIONS
Preamble
1. Chapter 1 – General introduction
1.1 The Phylum Cnidaria
1.1.1 Biodiversity
1.1.2 Ecological importance
1.1.3 General anatomy
1.1.4 Cellular anatomy
1.2 Physiological properties of cnidarians
1.2.1 Diversity of natural fluorescence
1.2.2 Cnidarian longevity and regeneration
1.2.3 Environmental adaptation
1.3 Our cnidarian study model: the sea anemone Anemonia viridis
1.4 Thesis objectives
2. Chapter 2 – In vivo studies of phenotypic plasticity
2.1 Introduction
2.1.1 Stress response: general context
2.1.2 Ocean acidification
2.2 Impact of ocean acidification on marine organisms
2.2.1 Impact of ocean acidification on calcification
2.2.2 Ocean acidification and carbon-concentrating mechanisms
2.3 Role of phenotypic plasticity in the response to OA
Abstract
Introduction
Materials and Methods
Results
Discussion
References
Supplementary materials
2.4 Impact of multiple stressors in cnidarians
Material and Methods
Results
Discussion
2.5 Conclusions
3. Chapter 3 – Development of a new in vitro cellular tool for the study of cnidarian phenotypic plasticity
3.1 Cnidarian cellular phenotypic plasticity
3.2 Vertebrate cell culture
3.2.1 Origin and contribution of vertebrate cell cultures
3.2.2 Diversity of vertebrate cell culture methodologies
3.3 Invertebrate cell cultures
3.3.1 Terrestrial cell cultures
3.3.2 Marine cell cultures
3.4 Cnidarian cell culture development
3.4.1 State of the art
3.4.2 Anemonia viridis cell culture establishment
3.5 Characterization and validation of cnidarian primary cell culture
Introduction
Materials and Methods
Results
Discussion
References
Supplementary materials
3.6 Further research on diversification of cell culture
3.6.1 In vitro primary cell culture assays from separated monolayers: epiderm vs. gastroderm .
3.6.2 Hanging drop culture assays for isolation and cultivation of cnidarian pluripotent cells .
3.7 Conclusions
4. Chapter 4 – General conclusions and perspectives
5. References