Organic fluorogenic or fluorescent probes for glutathione

For more info about our services contact : help@bestpfe.com

Table of contents

1. INTRODUCTION
2. THEORETICAL PART
2.1. Encapsulation of glutathione-selective fluorogenic probes
2.1.1. Biochemistry of glutathione
2.1.2. Functions of glutathione
2.1.3. Significance of glutathione in medicine and pharmacy
2.1.4. Analytical methods for glutathione detection and determination
2.1.5. In situ methods for glutathione and glutathione redox state imaging
2.1.5.1. Organic fluorogenic or fluorescent probes for glutathione
2.1.5.2. Redox-sensitive fluorescent proteins
2.1.5.3. Fluorescence nanoparticle probes
2.2. Encapsulation of salmon calcitonin
2.2.1. Physiologic action and clinical use of calcitonin
2.2.2. Structural properties and stability of calcitonin
2.2.3. Pharmaceutical preparations, side effects and pharmacokinetics
2.2.4. Novel calcitonin delivery systems and routes of administration
2.2.4.1. Parenteral sustained release calcitonin delivery systems
2.2.4.2. Oral calcitonin delivery systems
2.2.4.3. Other alternative routes of calcitonin administration
3. OBJECTIVES OF THE THESIS
4. EXPERIMENTAL PART
4.1. Encapsulation of glutathione-selective fluorogenic probes
4.1.1. Materials
4.1.2. Methods
4.1.2.1. Preparation of the probe-loaded nanoparticles
4.1.2.2. Characterization of the probe-loaded nanoparticles
4.1.2.3. In vitro release of the probe from nanoparticles
4.1.2.4. HPLC system and operating conditions
4.1.2.5. Pre-column derivatization of NDA
4.1.2.6. Study on NDA stability at various pH
4.1.2.7. Cell culture, probe loading and extraction conditions
4.1.2.8. Protein determination by the bicinchoninic acid method
4.1.2.9. Cytotoxicity studies
4.2. Encapsulation of salmon calcitonin
4.2.1. Materials
4.2.2. Methods
4.2.2.1. Preparation of salmon calcitonin-loaded nanoparticles
4.2.2.2. Characterization of salmon calcitonin-loaded nanoparticles
4.2.2.3. Study on salmon calcitonin stability
4.2.2.4. Study on salmon calcitonin adsorption to blank nanoparticles
4.2.2.5. In vitro salmon calcitonin release from nanoparticles
4.2.2.6. HPLC assay for salmon calcitonin analysis
4.2.2.7. DSC study
4.2.2.8. In vivo studies
4.2.2.9. Determination of salmon calcitonin in rat serum by ELISA
4.2.2.10. Spectrophotometric determination of calcium (II) in rat serum
4.2.2.11. Statistical analysis
5. RESULTS
5.1. Encapsulation of glutathione-selective fluorogenic probes
5.1.1. Validation of the HPLC method
5.1.2. Characterization of the probe loaded-nanoparticles
5.1.3. Loading of cells with NDA
5.1.4. Cytotoxicity of nanoparticles and NDA
5.2. Encapsulation of salmon calcitonin
5.2.1. Method validation of salmon calcitonin determination by HPLC
5.2.2. Method validation of salmon calcitonin determination by ELISA
5.2.3. In vitro characterization of calcitonin-loaded nanoparticles
5.2.4. Calcitonin in vitro stability and release from nanoparticles
5.2.5. In vivo studies
5.2.6. DSC study
6. DISCUSSION
6.1. Encapsulation of glutathione-selective fluorogenic probes
6.2. Encapsulation of salmon calcitonin
7. CONCLUSIONS
7.1. Encapsulation of glutathione-selective fluorogenic probes
7.2. Encapsulation of salmon calcitonin
8. REFERENCES