Photobleaching of sensitizers

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Table of contents

I GENERAL INTRODUCTION
II INTRODUCTION
II.1. HISTORY AND CLINICAL APPLICATIONS OF PHOTODYNAMIC THERAPY
II.2. PHOTOSENSITIZATION MECHANISMS
2. 1. Pathways of molecular excitation and deactivation
2. 2. Mechanisms of photosensitized reactions
2.2.1. Types I photosensitization mechanism
2.2.2. Types II photosensitization mechanism
2. 3. The properties of an ideal photosensitizer
2. 4. Tetraphenylchlorin series photosensitizers
2. 4. 1. The 5,10,15,20-tetrakis(m-hydroxyphenyl)chlorin
2. 5. Cells and tissue damage effects of PDT
2. 5. 1. Vascular shutdown and inflammation
2. 5. 2. Direct cell destruction
II.3. PHOTOPHYSICAL AND PHOTOCHEMICAL PROPERTIES OF SENSITIZERS
3. 1. Photobleaching of sensitizers
3. 1. 1. Parameters effecting photobleaching: aggregation state, pH, ionic strength and oxygen concentration
3. 2. Effect of aggregation state on photophysical and photochemical properties of sensitizers
3. 3. Photophysical properties of porphyrinoid sensitizer non-covalently bound to proteins
3. 4. Electronic structure of porphyrinoid photosensitizers
II.4. PHOTOSENSITIZERS INTERACTIONS WITH PLASMA PROTEINS
4. 1. Pharmacokinetics of sensitizers
4. 2. Kinetic and equilibrium characteristics of sensitizers interactions with proteins
4. 2. 1. Mechanisms of sensitizers redistribution between plasma proteins
4. 2. 1. 1. Collision mechanism
4. 2. 1. 2. Redistribution through the aqueous phase
4. 2. 2. Thermodynamics of sensitizers redistribution between plasma proteins. Eyring theory
II.5. INTRACELLULAR LOCALISATION OF PHOTOSENSITIZERS
5. 1. Techniques to study sensitizer intracellular localisation and aggregation state
5. 1. 1. Confocal laser scanning fluorescence microscopy
5. 1. 2. Fluorescence lifetime imaging microscopy (FLIM)
5. 2. Sub-cellular localisation and dynamics of sensitizers during PDT
5. 2. 1. Sites of sub-cellular localization of hydrophilic and hydrophobic sensitizers
5. 2. 2. Relocalisation of sensitizers upon irradiation
III OBJECTIVES
IV RESULTS
IV. 1. MTHPC-based photodynamic treatment in vivo
IV. 2. Investigation of mTHPC interactions with plasma proteins.
IV. 3. Redistribution of mTHPC from plasma proteins to model membranes
IV.4. Calculation of quantum yield of MCF-7 cells inactivation by mTHPC-PDT: influence of incubation time and sensitizers localization
IV.5. Theoretical and experimental study of the effects of solvent on the electronic structure of tetrapyrrole compounds: application for the determination of the structure of aggregates
V GENERAL DISCUSSION
VI CONCLUSION AND PERSPECTIVES