Plant and seeds of Salvia Hispanica L. as sources of biologically active substances

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Table of contents

CHAPTER I. LITERATURE REVIEW
1.1 ROLE OF LIPIDS IN BIOLOGICAL PROCESSES
1.2 PLANT AND SEEDS OF SALVIA HISPANICA L. AS SOURCES OF BIOLOGICALLY ACTIVE SUBSTANCES
1.3 COMPOSITION OF CHIA SEEDS
1.4 CHIA SEEDS LIPID FRACTION
1.5 CHIA SEED OIL COMPARED TO OTHER VEGETABLE OILS RICH IN UNSATURATED FATTY ACIDS
1.6 EFFECT OF CHIA SEED LIPIDS ON HEALTH AND DISEASE
1.7 METHODS OF CHIA SEED LIPIDS EXTRACTION
1.8 LIPID-BASED MICRO- AND NANOVESICLES AND DRUG DELIVERY
1.9 MAIN OBJECTIVE AND SPECIFIC AIMS OF THESIS RESEARCH
CHAPTER II. MATERIALS AND METHODS
2.1 MATERIALS
2.1.1 CELLS
2.2 METHODS
2.2.1 PHYSICO-CHEMICAL ANALYSIS OF ORURO CHIA SEEDS
2.2.2 CHARACTERIZATION OF CHIA SEED LIPID FRACTION
2.2.3 NANOPARTICLES PREPARATION
2.2.4 NANOPARTICLES SIZE, POLYDISPERSITY INDEX AND ZETA-POTENTIAL MEASUREMENTS
2.2.5 TRANSMISSION ELECTRON MICROSCOPY
2.2.6 STABILITY OF NANOPARTICLES
2.2.7 LONG-TERM STUDIES OF OBTAINED NANOPARTICLES
2.2.8 PHOSPHOLIPID DETERMINATION IN NANOLIPOSOMES AND NANOEMULSIONS
2.2.9 СYTOTOXICITY OF OBTAINED CHIA-BASED NANOLIPOSOMES AND NANOEMULSIONS
2.2.10 ANTIOXIDANT STUDIES OF CHIA-DERIVED NANOPARTICLES
2.2.11 STATISTICAL ANALYSIS
CHAPTER III. NANOLIPOSOMES AND NANOEMULSIONS BASED ON CHIA SEED LIPIDS: PREPARATION AND CHARACTERIZATION
ABSTRACT
3.1 INTRODUCTION
3.2 RESULTS
3.2.1 CHIA SEED LIPID EXTRACTION
3.2.2 CHARACTERIZATION OF CHIA SEED LIPID FRACTION
3.2.3 PHYSICOCHEMICAL CHARACTERIZATION OF NANOEMULSIONS ANDn NANOLIPOSOMES
3.3 DISCUSSION
3.4 CONCLUSIONS
CHAPTER IV. CYTOTOXICITY STUDIES OF NANOLIPOSOMES AND NANOEMULSIONS OBTAINED FROM CHIA SEED LIPIDS IN LIVER AND BRAIN CELLS
4.1 INTRODUCTION
4.2 RESULTS AND DISCUSSION
4.2.1 HEPA 1-6 CELL LINE
4.2.2 ASTOCYTES CELL LINES
4.2.3 PRIMARY CULTURE OF CORTICAL NEURONS
4.3 CONCLUSIONS
CHAPTER V. ADDITIONAL STUDIES
5.1 LONG-TERM STORAGE OF CHIA-DERIVED NANOLIPOSOMES AND NANOEMULSIONS
5.2 ANTIOXIDATIVE PROPERTIES OF NANOLIPOSOMES AND NANOEMULSIONS OBTAINED FROM CHIA SEED LIPIDS
CHAPTER VI. THESIS RESEARCH CONCLUSIONS AND PERSPECTIVES 
REFERENCES