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Table of contents
CHAPTER I. INTRODUCTION
I.1. REGULATION OF CARBON PARTITIONING IN THE PLANT AND RHIZODEPOSITION
I.2. RHIZODEPOSITS: SOURCE OF ENERGY AND INFORMATION FOR MICROORGANISMS
I.3. PHOTOSYNTHATES ALLOCATION TOWARDS ROOT INFECTING AND FREE LIVING SYMBIONTS: AM FUNGI AND PROTOZOA
I.4. PROTOZOA – ARBUSCULAR MYCORRHIZAL FUNGI INTERACTIONS
I.5. OBJECTIVES
CHAPTER II. THE IMPACT OF PROTOZOA ON PLANT NITROGEN UPTAKE AND MORPHOLOGY VARIES WITH PLANT SPECIES
II.1. INTRODUCTION
II.2. MATERIALS AND METHODS
II.2.1. Plants, microcosms and incubation procedure
II.2.2. Preparation of microcosms and soil
II.2.3. Inoculation with bacteria and protozoa
II.2.4. Plant transfer and cultivation
II.2.5. Harvesting and analytical procedures
II.2.6. Statistical analysis
II.3. RESULTS
II.3.1. Plant growth as affected by Acanthamoeba castellanii
II.4. DISCUSSION
II.4.1. Conclusions
ACKNOWLEDEGMENTS
REFERENCES
CHAPTER III. EFFECTS OF PROTOZOA ON PLANT NUTRITION AND CARBON ALLOCATION DEPENDS ON THE QUALITY OF LITTER RESOURCES IN SOIL
III.1. INTRODUCTION
III.2. MATERIAL AND METHODS
III.2.1. Microcosms
III.2.2. Plants and incubation conditions
III.2.3. Preparation of 15N labelled plant litter
III.2.4. 13CO2 pulse labelling of plants
III.2.5. Plant and soil analyses
III.2.6. Analysis of the 13C/12C and 14N/15N ratios of soil and plant samples
III.2.7. PLFA patterns and lipid stable isotope probing
III.2.8. Counting of amoebae
III.2.9. Quantification of microbial N
III.2.10. Statistical analyses
III.3. RESULTS
III.3.1. Plant biomass, total C and N
III.3.2. Plant and microbial 15N and 13C enrichment
III.3.3. 13C enrichment of plant organs
III.3.4. 13C enrichment of belowground respiration
III.3.5. Phospholipid fatty acids
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III.3.6. δ13C signatures of PLFAs
III.4. DISCUSSION
III.4.1. Conclusions
ACKNOWLEDGEMENTS
REFERENCES
CHAPTER IV. PROTOZOA (ACANTHAMOEBA CASTELLANII) AND ARBUSCULAR MYCORRHIZAL FUNGI (GLOMUS INTRARADICES) MEDIATE THE PARTITIONING OF CARBON AND THE AVAILABILITY OF NITROGEN FOR PLANTAGO LANCEOLATA
IV.1. INTRODUCTION
IV.2. MATERIAL AND METHODS
IV.2.1. Microcosms, soil and microorganisms
IV.2.2. Plant preparation and growth conditions
IV.2.3. 13CO2 pulse labelling and quantification of 13C respiration of the belowground compartment
IV.2.4. Plant harvest and soil sampling
IV.2.5. Total C, N and isotope (13C/12C and 14N/15N) analyses of soil and plant samples
IV.2.6. Soil soluble mineral N (Nmin) concentration and microbial biomass
IV.2.7. Size and activity of the soil microbial community
IV.2.8. Microbial community structure
IV.2.9. Statistical analyses
IV.3. RESULTS
IV.3.1. Plant biomass and shoot-to-root ratio
IV.3.2. Total N, atom% 15N and total 15N in Plantago lanceolata
IV.3.3. Belowground respiration
IV.3.4. Soluble Nmin soil
IV.3.5. Microbial biomass, activity and community structure
IV.4. DISCUSSION
IV.4.1. Conclusions
ACKNOWLEDGEMENTS
REFERENCES
CHAPTER V. PROTOZOA AND ARBUSCULAR MYCORRHIZA COMPLEMENT EACH OTHER IN PLANT NITROGEN NUTRITION FROM A NUTRIENT PATCH
V.1. INTRODUCTION
V.2. MATERIAL AND METHODS
V.2.1. Microcosms and labelling procedure
V.2.2. Analytical procedures
V.2.3. Statistical Analysis
V.2.4. Results and Discussion
ACKNOWLEDGEMENTS
REFERENCES
CHAPTER VI. GENERAL DISCUSSION
