Root-knot nematodes

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Table of contents

INTRODUCTION
1. Generalities about Nematodes
1.1. Nematode structure
1.2. Nematode lifestyles
1.3. Nematode systematics
2. Plant-parasitic nematodes
3. Root-knot nematodes
3.1. Reproduction mode
3.2. Root-knot nematode control
3.3. RKN life cycle
4. Giant cells: formation and main characteristics
4.1. Cell cycle and cytoskeleton reorganization during giant cell formation.
4.2. Importance of the metabolism in GC
5. RKN effectors
5.1. Identification of RKN effectors.
5.2. Functional analysis of effectors
6. Transcriptomic approach to identify nematode effectors
7. Objectives
CHAPTER 1: Identification of Parasitism Effectors Expressed During Plant Infection from the Transcriptome of Meloidogyne incognita
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References
Supplementary data chapter 1
CHAPTER 2: Transcriptome Profiling of the Root-Knot Nematode Meloidogyne enterolobii During Parasitism and Identification of Novel Effector Proteins
Introduction
Material and methods
Results
Discussion
Acknowledgements
References
GENERAL DISCUSSION, CONCLUSIONS AND PERSPECTIVES
1. Identification of parasitism effectors expressed during plant infection from the transcriptome of Meloidogyne incognita.
2. Transcriptome profiling of the root-knot nematode Meloidogyne enterolobii during parasitism and identification of novel effector proteins
3. Toward new resistance strategies against RKN.
References
ANNEXES
Annex 1: In situ Hybridisation protocol for localisation of gene expression in nematode
Annex 2: siRNA soaking protocol and resistance test
Annex 3 : Curriculum vitae