Storage Proteins (Prolamins or non-soluble)

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Table of contents

CHAPTER 1: INTRODUCTION
1 WHEAT
1.1 ORIGIN
1.2 EVOLUTIONARY HISTORY
1.3 WHEAT GENOME
1.4 WHEAT CLASSIFICATION
1.5 WHEAT PLANT ANATOMY
1.6 WHEAT GRAIN STRUCTURE
1.6.1 Peripheral Layers (PL)
1.6.2 Endosperm (ESM)
1.6.3 Embryo
2 DEVELOPMENT OF WHEAT PLANT AND GRAIN
2.1 GERMINATION TO THE END OF VEGETATIVE GROWTH
2.2 FLOWERING AND FERTILIZATION
2.3 GRAIN DEVELOPMENT
2.3.1 Grain Growth
2.3.2 Grain Filling
2.3.3 Grain dehydration-maturation stage
3 WHEAT GRAIN CONTENTS (COMPOSITION)
3.1 NON PROTEIN CONTENTS
3.2 PROTEIN CONTENTS
3.2.1 Metabolic Proteins (Non-prolamins or Soluble)
3.2.2 Storage Proteins (Prolamins or non-soluble)
3.3 IMPACT OF WHEAT PROTEINS ON ITS QUALITY
4 PROTEOMICS TO STUDY EXPRESSION OF CANDIDATE GENES
4.1 PROTEOMICS
4.2 QUANTITATIVE PROTEOMICS
4.2.1 Relative quantification
4.2.2 Absolute Quantification
4.3 MASS SPECTROMETRY
4.3.1 MALDI-TOF (Matrix-Assisted Laser Desorption -Time of flight)
4.3.2 LC-MS (Liquid chromatography mass spectrometry)
4.3.3 Tandem mass spectrometry (MSMS or MS²)
5 TRANSCRIPTOMICS TO STUDY EXPRESSION OF CANDIDATE GENE
5.1 METHODS TO STUDY TRANSCRIPTIONAL EXPRESSION
5.1.1 OPEN SYSTEMS
5.1.2 CLOSED SYSTEMS
5.2 WHEAT TRANSCRIPTOMICS STUDIES
6 INTEGRATION OF PROTEOMICS AND TRANSCRIPTOMICS DATA
CHAPTER 2 (ARTICLE 1): PROTEOMIC ANALYSIS OF PERIPHERAL LAYERS DURING WHEAT (TRITICUM AESTIVUM L.) GRAIN DEVELOPMENT
CHAPTER 3 (ARTICLE 2): EXPRESSION PROFILING OF STARCHY ENDOSPERM METABOLIC PROTEINS AT TWENTY-ONE STAGES OF WHEAT GRAIN DEVELOPMENT
CHAPTER 4 (ARTICLE 3): AN ATTEMPT TO INTEGRATE PROTEOME AND TRANSCRIPTOME DATA: APPLICATION TO CARBOHYDRATE METABOLISM OF WHEAT GRAIN DEVELOPMENT
CHAPTER 5: COMPARATIVE PROTEOME STUDY OF PERIPHERAL LAYERS AND STARCHY ENDOSPERM DURING GRAIN DEVELOPMENT
1METABOLIC PROTEINS IDENTIFIED IN GRAIN TISSUES
2 EXPRESSION PROFILES OF PL AND ESM PROTEINS
3 FUNCTIONAL PROFILES OF PL AND ESM
3.1 METABOLISM
3.2 GENETIC INFORMATION AND PROCESSING
3.3 ENVIRONMENTAL INFORMATION AND PROCESSING
3.4 STORAGE PROTEINS (SP)
3.5 STRESS/ DEFENSE (SD)
3.6 REDOX HOMEOSTASIS
CONCLUSION AND PERSPECTIVES
REFERENCES