The Bioinformatic field

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Table of contents

1.! Introduction
2.! Materials and Methods
2.1.! Bioinformatics
2.1.1.! Primers
2.2.! Inoculation Procedure
2.3.! Plasmid Isolation
2.4.! Site-Directed Mutagenesis
2.4.1.! MUT1-MO2.1
2.4.2.! MUT2-MO2.1
2.4.3.! Mutagenesis PCR
2.5.! Blue/White Screening
2.6.! Subculture of Colonies
2.7.! Protein Isolation
2.8.! Protein Concentration Using Bradford Assay
2.9.! Protein Activity
2.9.1.! Coagulation Activity Using Clay Solution
2.9.2.! Antimicrobial Activity
2.9.3.! Disc Diffusion Method
3.! Results and Discussion
3.1.! Bioinformatics
3.2.! Mutagenesis PCR
3.3.! Coagulation Activity
3.3.1.! MUT1-MO2.1
3.4.! Antimicrobial activity
3.4.1.! Disk Diffusion Method
3.5.! MIC
3.6.! Protein Concentration
3.7.! Kinetics
3.7.1.! MUT1-MO2.1
3.7.2.! MUT2-MO2.1
4.! Conclusions
5.! Acknowledgement
6.! References