The evaluation of Folic acid stability

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Table of contents

Chapter I: General Overview 
[Cancer, Photodynamic Therapy and Drug Targeting]
1.0 General Introduction
1.1 Cancer and Its Therapeutics
1.1.1 General Overview
1.1.2 Development of Cancer: Cells, Genes and Mutations
1.1.3 Angiogenesis: Background review
1.1.4 Angiogenesis: The process
1.1.5 Neuropilin-1 receptor and cancer
1.1.6 Folate receptor and cancer
1.1.7 Treatment approach for cancer
A. Surgery
B. Chemotherapy
C. Radiotherapy
D. Immunotherapy
E. Hormone therapy
F. Bone marrow transplant
1.1.7 Current Anti-angiogenesis Treatment
1.2 Photodynamic Therapy
1.2.1 Historical background of photodynamic therapy
1.2.2 Overview of PDT
1.2.3 Components of Photodynamic Therapy
A. Light
B. Oxygen
C. Photosensitizer
1.2.4 Classification of photosensitizers
1.2.5 Porphyrin: Historical Background and Current Advancement
A. Structure of porphyrin
B. Synthesis of Porphyrin
C. Photophysical characteristics
1.2.6 Photodynamic action in vivo
1.3 Targeted Cancer Therapy: A Strategy in Improving the Delivery of Photosensitizer
1.3.1 Improving PS Delivery for PDT
1.3.2 Targeting with peptide
A. DKPPR peptide
B. Target application of DKPPR and KDKPPR in PDT
1.3.3 Targeting with folic acid
1.4 Objectives of Study
Chapter II: The Evaluation of Folic Acid Stability: As Free Molecule and as Conjugate for PDT Application 
2.1 Introduction
2.1.1 Folic acid
2.1.2 Folic acid in cancer therapy
2.1.4 Conjugation with a Model Photosensitizer
2.1.5 Experimental approach: Design of Experiment
2.1.6 Statistical analysis
2.2 Results and Discussion
2.2.1 The solubilisation of FA and PS-FA
2.2.2 The evaluation of Folic acid stability
2.2.3 The evaluation of PS-FA conjugate stability
2.2.4 Statistical analysis
2.3 Conclusion and Future Perspective
Chapter III: Modification of KDKPPR Peptide to Investigate the Effect on its Binding on NRP Receptot
3.1 Introduction
3.1.1 Peptides
3.1.2 Peptides and Solid-phase peptide synthesis
3.1.3 Peptides and drug development
3.1.4 KDKPPR peptide
3.1.5 Peptide modifications performed in this study
A. Alanine-scanning
B. Replacement of K2 lysine with arginine (KDRPPR)
C. Retro, inverso and retro-inverso peptides
3.1.6 ELISA competitive binding assay
3.2 Results and Discussion
3.2.1 NMR analysis
3.2.2 ELISA competitive binding assay
3.3 Conclusion and Future Perspective
Chapter IV: Synthesis of Porphyrin (P1COOH) and DKPPR Peptide Platform through Click Chemistry
4.1 Introduction
4.1.1 Click chemistry
4.1.2 The advantages and disadvantages of click chemistry
4.1.3 Porphyrin-based click chemistry
4.1.4 Microwave-assisted click chemistry
4.1.5 Peptides in click chemistry
4.1.6 Synthesis plan
4.2 Results and Discussion
4.2.1 The synthesis of platform building blocks
4.2.2 Building porphyrin blocks through click chemistry
4.2.3 Photophysical properties
A. Absorption
B. Fluorescence
C. Singlet oxygen
4.2.4 Building DKPPR blocks through click chemistry
4.2.5 Building the Porphyrin-DKPPR platform through click chemistry
4.2.6 ELISA Competitive Assay
4.3 Conclusion and Future Perspective
Conclusion
References