(Downloads - 0)
For more info about our services contact : help@bestpfe.com
Table of contents
Chapter I. Introduction
I.A. The nervous system
I.A.1. Structure and evolution of the nervous system
I.A.2. Cells in the nervous systems
I.A.3. The neuronal cytoskeleton
I.B. Axon branching in the developing vertebrate CNS
I.B.1. Collateral branching
I.B.2. Branching through growth cone splitting
I.C. Axon turning through growth cone steering
I.C.1. The cytoskeletal dynamics of turning
I.C.2. Signaling mechanisms underlying turning
I.C.3. Conclusion
I.D. Studying neuronal branching in vitro: a review of different systems
I.D.1. Studying neuronal cell development in vitro
I.D.2. Microengineering tools to study neuronal growth in vitro
I.D.3. Application of microengineering tools to the study of neuronal branching in vitro
Chapter II. Materials and Methods
II.A. Introduction
II.B. Primary cell cultures
II.B.1. Neuronal cell cultures
II.B.2. Fixation
II.B.3. Immunofluorescence
II.C. Patterned substrates
II.C.1. Static patterns
II.C.2. Dynamic patterns
II.D. Microscopy observations
II.D.1. Time-lapse experiments
II.D.2. Fixed cells
II.E. Analysis methods
II.E.1. Growth cone analysis
II.E.2. Branching probability and measure of branch length
II.E.3. Time-lapse analysis
II.E.4. Statistical tests
Chapter III. Results
III.A. Static micropatterning to study neuronal branching
III.A.1. Introduction
III.A.2. Methodology
III.A.3. Analysis of growth cone morphology
III.A.4. Static analysis of branching
III.A.5. Dynamic analysis of neuronal branching on static patterns
III.A.6. Conclusion
III.B. Dynamic patterning to study neuronal branching
III.B.1. Context and objective of the project
III.B.2. Methodology and results
III.B.3. Conclusion
Chapter IV. Discussion
IV.A. Actin based exploration of the GC microenvironment
IV.B. Selection of direction by microtubules
IV.C. Axons prefer going straight
IV.D. Conclusion
Bibliography
