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Table of contents
1 INTRODUCTION
2 LITERATURE REVIEW
2.1 MICROBIAL COMMUNITIES IN THE ANAEROBIC DIGESTION PROCESS
2.1.1 The different steps of anaerobic digestion
2.1.2 Abiotic parameters influencing anaerobic digestion
2.2 MOLECULAR TOOL TO STUDY MICROBIAL COMMUNITIES IN THE ANAEROBIC DIGESTION PROCESS
2.3 MICROBIAL DIVERSITY AND STRUCTURE
2.3.1 Concept of microbial diversity
2.3.2 Diversity measurements
2.3.3 Community distances
2.4 MICROBIAL INTERACTIONS
2.5 MICROBIAL DIVERSITY AND ECOSYSTEMS FUNCTION
2.5.1 Relationship between microbial diversity and ecosystems function
2.5.2 Engineering microbial diversity-function experiments
2.6 COMMUNITY ASSEMBLY
3 THESIS OBJECTIVES
4 MATERIAL AND METHODS
4.1 INOCULUM AND SUBSTRATE CHOICE
4.2 DESIGN OF LAMACS
4.3 PRESSURE DATA ANALYSIS
4.4 ANALYTICAL METHODS
4.4.1 Biochemical analyses
4.4.2 Biological analyses
4.5 BIOMASS PREPARATION AND INOCULATION
4.6 STATISTICAL ANALYSES
4.7 SUMMARY OF EXPERIMENTS PERFORMED
5 MULTIPLEXED CHEMOSTAT SYSTEM FOR QUANTIFICATION OF BIODIVERSITY AND ECOSYSTEM FUNCTIONING IN ANAEROBIC DIGESTION
5.1 ABSTRACT
5.2 INTRODUCTION
5.3 RESULTS
5.3.1 Range of operating conditions of the multiplexed chemostats
5.3.2 Sensitivity of performance measurement
5.3.3 Long-term operation of the multiplexed chemostats
5.4 DISCUSSION
5.5 ADDITIONAL DATA
6 SUBSTRATE AND INOCULUM CHOICE AFFECT ASSEMBLY OF FUNCTIONALLY REDUNDANT ANAEROBIC COMMUNITIES
6.1 INTRODUCTION
6.2 RESULTS
6.2.1 Reactor operation
6.2.2 Influence of substrate and community assembly on ecosystem functioning
6.2.3 Relating microbial diversity to ecosystem functioning
6.2.4 Relating microbial community structures to ecosystem functioning
6.2.5 Coalescence of mixed communities
6.3 DISCUSSION
6.4 CONCLUSION
6.5 ADDITIONAL DATA
7 GENERAL CONCLUSION
8 PERSPECTIVES
9 REFERENCES
