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Table of contents
INTRODUCTION
VITAMIN C – ASCORBIC ACID
HISTORICAL BACKGROUND: LET US GO BACK A BIT IN TIME
CHEMICAL DESCRIPTION OF VITAMIN C
ASCORBIC ACID METABOLISM
i. Biosynthesis
ii. Recycling: redox state
iii. Degradation
REGULATION: VARIABILITY IN PLANTA
i. Genetic variability
ii. Spatiotemporal variability
iii. Environmental impact
iv. GGP/VTC2: the key enzyme
BIOLOGICAL ROLES IN PLANTS
i. Plant development: from seed to fruits
ii. Photosynthesis
iii. Oxidative stress (Post-harvest)
iv. Pathogenic infection
TOMATO – MORE THAN A FRUIT, A MODEL
FROM ORIGINS TO DOMESTICATION
A MODEL SPECIE
i. Agro-economic importance
ii. Food losses from field to plate: Post-harvest injuries
i. Genetic tools
MICROTOM ASCORBATE MUTANTS
i. EMS mutant library
ii. Reverse genetic: Ascorbate-deficient mutants
iii. Forward genetic: Ascorbate-enriched mutants
LIGHT – THE DARK SIDE OF ASCORBATE
DESCRIPTION
i. Some physical aspects of light
ii. Light and plants
i. Light and ascorbate
i. Photo-Sensor Systems
PASLOV PROTEINS
OBJECTIVES OF THE THESIS WORK
RESULTS
PART 1: A BLIND REGULATOR
THE KEY ENZYME GGP REGULATES ASCORBIC ACID CONTENT THROUGH ITS INTERACTION WITH A NEW CLASS OF PHOTORECEPTOR IN TOMATO.
Introduction
Results
Discussion
Materials and Methods
Supplemental
References
PART 2: STERILE DEREGULATION
KNOCKING-OUT SLGGP RETRO-REGULATION LEADS TO HIGH ASCORBATE ACCUMULATION RESULTING IN A DEFECT OF THE REPRODUCTIVE SYSTEM IN TOMATO.
Introduction
Results
Discussion
Materials & Methods
Supplemental
References
PART 3: CHILLING STRESS
ANTIOXIDANT POTENTIAL IN POSTHARVEST FRUIT QUALITY.
Introduction
Results
Discussion
Materials and Methods
CONCLUSION
ANNEXES
Primers and vectors used for GATEWAY® cloning
Agrobacterium-mediated stable transformation of tomato
Biolistic transformation of onion epidermis
Agroinfiltration of Nicotiana Benthamiana leaves
Setting of the light experiment in the growth chamber.
CRISPR Cas9
Optogenetic assay
Ascorbic acid measurement
REFERENCES
