Transcription factor regulation

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Table of contents

Chapter 1 Introduction
Gene expression and RNA Polymerase
Controling gene expression: the role of transcription factors
Mechanisms of gene repression
Transcription factor regulation
Transcriptional dynamics
The use of fluorescence microscopy
Chapter 2 Results
Dynamic changes in RNA Polymerase 2 processivity during transcriptional activation
Discussion
Chapter 3 Other publications
mRNA degradation by Xrn1 stimulates transcription
Short exposure to the DNA intercalator DRAQ5 dislocates the transcription machinery and induces cell death
The In Vivo Kinetics of RNA Polymerase II Elongation During Co-Transcriptional Splicing
Imaging transcription in living cells
Mouaikel-Libri
Chapter 4 Methodology Improvements
Live Cell Imaging
FISH
Hyperresolution work in progress
Chapter 5 New types of data require novel analysis methodologies
Chapter 6 Materials and methods
Cell Culture
Transfection
Generation of derived cell-lines
Table 1 : Generated cell lines
Luciferase
FISH
Chemical coupling of FISH probes
Immunofluorescence.
Fixed cell imaging
Live cell imaging
Chapter 7 Bibliography