Zebrafish sex differentiation

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Table of contents

ABSTRACT
SAMMANFATTNING
1. INTRODUCTION
1.1 SIRT1
1.2 SIRT1 in Zebrafish
1.3 EX-527
1.4 Zebrafish sex differentiation
1.5 Aim of the study
2. MATERIAL AND METHODS
2.1 Zebrafish maintenance and breeding
2.2 Molecular phylogenetic study
2.3 Computational modeling
2.4 Exposure of zebrafish
2.5 RNA extraction and qRT-PCR
2.6 Western Blot analysis
2.7 Statistical analysis
3. RESULT AND DISCUSSION
3.1 The catalytic domain of sirt1 is conserved
3.2 Exposure to EX-527 down-regulates sex related genes in larval and juvenile zebrafish
3.3 Exposure to EX-527 up-regulates apoptotic related genes in larval and juvenile zebrafish
3.4 Exposure to EX-527 causes malformations and higher mortality
3.5 EX-527 exposure does not affect sex ratio
4. CONCLUSION
AKNOWLEDGEMENTS
REFERENCES
SUPPLEMENTARY DATA