ACCURACY OF THE BROMOCRESOL GREEN METHOD FOR ALBUMIN DETERMINATION

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INTRODUCTION

White rhinoceros that survive poaching attempts require veterinary care for their injuries. Clinical pathology plays an important role in the initial evaluation and ongoing monitoring of these animals; however, the delay between blood sampling and subsequent analysis at a reference laboratory can be up to 24 hours (9). These compromised animals generally need to be chemically immobilized each time a veterinary procedure (including blood sampling) is performed, which is associated with the risk of respiratory depression, hypertension and renarcotisation (10).

Analyser

The POCA evaluated in this study was the IDEXX VetTest dry slide chemistry analyser (IDEXX Laboratories, Inc., Westbrook, ME, USA), which is described in more detail in Chapter 1. The choice of this analyser was based on the donation of a VetTest to Saving the Survivors specifically for use in injured rhinoceros. The reaction slides must be stored at -18°C and can be used directly from the freezer (39). A maximum of 12 slides can be used per run.

QC Validation

QC validation was performed using the CVs obtained from the long-term imprecision study. As the supplier of the QCM did not supply target values for this analyser, a useful estimate of bias for the purpose of QC validation could not be calculated and was set at zero for these calculations.

QC Strategy

The 2s or 3s control rule limits were calculated from the SDs of the original set of QCM measurements. The mean values from the original 20 measurements served as the target values. A protocol for future statistical QC was developed based on the selected control rules and limits for the chosen levels. Levey-Jennings charts were created for each analyte.

DEDICATION
ACKNOWLEDGEMENTS
CHAPTER 1: BACKGROUND
THE NEED FOR VETERINARY CARE OF THE WHITE RHINOCEROS
THE IDEXX VETTEST 8800: A POINT-OF-CARE CHEMISTRY ANALYSER
METHOD VALIDATION
STATISTICAL QUALITY CONTROL
PERFORMANCE IN THE FIELD
REFERENCE INTERVALS FOR CLINICAL CHEMISTRY
SERUM PROTEIN ELECTROPHORESIS
ACCURACY OF THE BROMOCRESOL GREEN METHOD FOR ALBUMIN DETERMINATION
CHAPTER 2: RESEARCH OBJECTIVES
CHAPTER 3: ANALYTICAL AND QUALITY CONTROL VALIDATION AND ASSESSMENT OF FIELD PERFORMANCE OF A POINT-OF-CARE CHEMISTRY ANALYSER FOR USE IN THE WHITE RHINOCEROS
SUMMARY
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION AND CONCLUSION
CHAPTER 4: METHOD COMPARISON AND GENERATION OF PLASMA BIOCHEMISTRY REFERENCE INTERVALS FOR THE WHITE RHINOCEROS ON A POINT-OF-CARE AND WET CHEMISTRY ANALYSER
SUMMARY
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CHAPTER 5: PROTEIN ELECTROPHORESIS IN WHITE RHINOCEROS
SUMMARY
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION AND CONCLUSIONS
CHAPTER 6: ACCURACY OF THE BROMOCRESOL GREEN METHOD FOR ALBUMIN MEASUREMENT
SUMMARY
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION AND CONCLUSIONS
CHAPTER 7: DISCUSSION AND CONCLUDING REMARKS
CHAPTER 8: FUTURE RESEARCH DIRECTIONS
REFERENCES

GET THE COMPLETE PROJECT
VALIDATION OF A POINT-OF-CARE CLINICAL CHEMISTRY ANALYSER, GENERATION OF CLINICAL CHEMISTRY REFERENCE INTERVALS AND EVALUATION OF SERUM PROTEIN ELECTROPHORESIS IN THE SOUTHERN WHITE RHINOCEROS, CERATOTHERIUM SIMUM SIMUM

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