Benefit of exercise practice during cancer cachexia

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Table of contents

INDEX
THE LIST OF ABBREVIATIONS
THE THESIS EXPLAINED
INTRODUCTION
1. Muscle mass homeostasis in the adult
1.1 Skeletal muscle regeneration
1.2 Muscle mass regulation.
2. Cancer effects on muscle mass
3. Exercise effect on cancer cachexia
3.1 Benefit of exercise practice during cancer cachexia.
3.2 Molecular pathway involved in exercise response
4. Serum response factor
4.1SRF expression
4.2SRF target genes
4.3 Regulation of SRF activity by Rho/actin/MRTFs pathway
4.4SRF Knock-out in skeletal muscle cells
OBJECTIVES
RESULTS.
5. C26 cell conditioned medium effects on C2C12 myotube
5.1 C26 cell conditioned medium blocks myostatin growth while mechanical stimulation counteracts this negative effect
5.2 Mechanical cues rescue the myogenic differentiation program via MRFs activation
6. C26 cell conditioned medium and mechanical cues affect myogenic differentiation via secreted factor by modulating secreted factors
6.1 Mechanical cues restore Activin A and Follistatin balance favouring myogenic differentiation
6.2 Follistatin is not sufficient to counteract myotube atrophy due to conditioned medium
7. SRF activity counteracts the negative effect of C26 cell conditioned medium on myogenic differentiation
7.1 Mechanical cues activate the Rho/actin/MRTFs pathway
7.2 MRTF-A translocation to the nucleus is stimulated by mechanical cues
7.3 SRF expression increases in presence of mechanical cues
7.4 A knock-down of SRF is sufficient to block the myogenic differentiation rescue associated to mechanical cues
DISCUSSION
MATERIAL AND METHODS
8. Cell culture
8.1 C26 conditioned medium protocol
8.2 Flexcell
9. Infection by adenovirus MRTF-A-GFP
10. Cell treatment by recombinant follistatin
11. SRF Knock-down
12. Immunofluorescence
PhD in Morphogenesis and Tissue Engineering
PhD in Physiology, physiopathology and therapeutics
13. RNA extraction, cDNA synthesis and Q-PCR
14. ELISA
15. Western Blot
15.1 Western Blot
15.2 Western Blot of F and G-actin
16. Imaging and quantification
17. Statistical analysis
REFERENCES