Seed Water Uptake

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Table of contents

Chapter 1 Introduction 
I. Seed Development and Composition
II. Dormancy and Control of Germination
III. Cellular Events During Germination
III.1. Seed Water Uptake
III.2. Respiration
III.3. RNA and Protein Synthesis
III.4. Cell Wall Modification and Cell Elongation
IV. Hormonal Regulation of Seed Dormancy and Germination
V. Reactive Oxygen Species Action in Seed Biology
V.1. ROS Production and Scavenging System
V.2. ROS in the Regulation of Dormancy Alleviation and Germination
V.3. ROS and Protein Oxidation
V.3.1. Carbonylation
V.3.2. Sulfur-centers oxidation
V.4. ROS in Cell Wall Loosening
VI. Objectives of the Thesis on Sunflower Seed Dormancy and Germination
Chapter 2 Cell wall loosening during dormancy alleviation in sunflower seeds
I. Introduction
II. Materials and Methods
II.1. Plant Material and Treatment
II.2. Germination Tests
II.3. Atomic Force Microscopy (AFM)
II.4. Cell Wall Monosaccharide Composition
II.5. Pectin Methyl-Esterase (PME) Activity
II.6. Immunofluorescence Studies and Histological Examination
II.7. Statistical Analysis
III. Results
III.1. Germination Assay
III.2. Wall Stiffness Assessment
III.3. Characterization of Cell Wall Monosaccharide Composition
III.4. PME Activity Measurements
III.5. In Situ Detection of Cell Wall Methylation
IV. Discussion
V. Conclusion
VI. Acknowledgements
VII. References
Supporting Information
Chapter 3 Proteomics of protein-bound methionine oxidation in sunflower seed 
I. Introduction
II. Materials and Methods
II.1. Seed Materials and Treatment Conditions
II.2. NAD(H), NADP(H), Glutathione and Ascorbate Pool Measurement
II.3. Protein Extraction
II.4. Protein Digestion
II.5. Diagonal Chromatography
II.6. LC-MS/MS Analysis
II.7. Data Processing
III. Results and Discussion
III.1. Germination Assay
III.2. Redox Metabolite Status
III.2.1. Redox treatments profile
III.2.2 Redox metabolites profile
III.3 Protein Identification and Expression Profiles among Different Treatments
III.4. Functions and Pathways Analysis of Differentially Expressed Proteins
III.4.1 Transcription and translation
III.4.2 Protein metabolism, binding and remodeling
III.4.3 Energy and metabolism
III.4.4 Stress response
III.4.5 Oxidation–reduction-related proteins
IV. Conclusion
V. Acknowlegments
Chapter 4 General conclusion